Czech

Not applicable.

Theoretical aspects of various experimental techniques used in molecular biotechnology research in GMO construction and their practical use.

Pass out the lectures of Molecular genetics I and II is recomended

Not applicable.

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Examination is written (test) and oral. The student had to proof the understanding of the subject.

924/5000
• The relationship between chemical production and biotechnology.
• DNA isolation and recombinant DNA technology.
• DNA cloning, polymerase chain reaction, PCR utilization in clinical and food diagnostics
• Cloning vectors and their use.
• Transformacfe - Transfer of foreign DNA into cells. Selection of cloned gene cells.
• Fusion proteins. Translational expression vectors. Enhancement of protein stability. Integration and shuttle vectors.
• Yeast expression systems. Systems using insect cells, mammalian expression systems.
• Biotechnological use of genetically modified micro-organisms.
• Transgenic plants and animal animals.
• Bioinformatics in gene biotechnology
• Use of bioinformatic approaches in the study of nucleic acids and proteins
• Gene therapy Diseases treatable by gene therapy. Vectors for gene therapy. Perspectives of gene therapy.
• Development, characterization and antibody production

Not applicable.

Lectures cover methodologies commonly used in molecular biotechnology in construction of transgenic organims and with the use of these organisms.

Attendance at lectures is not obligatory but it is recommended

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