Course detail
Advanced microscopic techniques in biology
FEKT-DBT3Acad. year: 2018/2019
Introduction to microscopy techniques and their use in biology. Fluorescence microscopy, dyes, their characterization and use. Histochemical, immunohistochemical and immunofluorescence. Microscopic methods for detection of cell organelles ( nucleus, nucleolus, endoplasmic reticulum, Golgi apparatus, mitochondria, cytoskeleton, lysosomes, vacuoles and other membrane structures). Microscopic detection cell function and apoptosis (programmed cell death). Detection ions, reactive oxygen and nitrogen. Nanoparticles and their use in microscopic techniques.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Course curriculum
2. Methods for the preparation of biological materials for various microscopic techniques and their specifics.
3. Fluorescence dyes used in microscopic techniques. Fluorescent dyes. Characterization, classification.
4. Histochemical, immunohistochemical and immunofluorescence techniques. Principles and applications.
5. Cell organelles, their structure and function I - using microscopic techniques.
6. Cell organelles, their structure and function II - using microscopic techniques.
7. Cell cycle and apoptosis. Microscopic detection of apoptosis (programmed cell death) as a complex process.
8. Microscopic detection of bioactive molecules (amines, thiols, carboxylic acids, proteins, etc.).
9. Microscopic detection of reactive oxygen and nitrogen.
10. Microscopic ion detection.
11. In situ hybridization.
12. Nanoparticles and their use in microscopic techniques
Work placements
Aims
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Časopis Microscopy Research and Technique (Wiley)
Hoppert, M. Microscopic Techniques in Biotechnology, Wiley, 2001
Recommended reading
Classification of course in study plans