Course detail

Modern methods in electrophysiology research

FEKT-DBT5Acad. year: 2018/2019

History and methods used in experimental cardiology. Isolated perfused Langendorff heart and its use for the isolation of cardiomyocytes. Methods for measuring of the membrane voltages and currents in different models. Electrocardiography and measuring the pressure in animal models. Registration of monophasic action potentials using various methods.

Language of instruction

Czech

Number of ECTS credits

4

Mode of study

Not applicable.

Guarantor

prof. MUDr. Marie Nováková, Ph.D.

Department

Department of Biomedical Engineering (UBMI)

Learning outcomes of the course unit

Not applicable.

Prerequisites

Not applicable.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Not applicable.

Course curriculum

1. History of experimental cardiology .
2. Methods of experimental cardiology - overview.
3. Isolated perfused Langendorff heart.
4. Cardiomyocytes.
5. Voltage clamp.
6. Patch clamp.
7. Electrocardiography of animal models.
8. The acquisition of monophasic action potentials via different methods.
9. The acquisition of pressure on isolated heart and the heart in situ.
10. Biochemical methods used in experimental cardiology.
11. Genetic and genomic studies.

Work placements

Not applicable.

Aims

The aim of the course is to provide an overview of the methodologies used in experimental cardiology, including an analysis of the obtained signals.

Specification of controlled education, way of implementation and compensation for absences

Not applicable.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Natale,A. Handbook of cardiac Electrophysiology, Informa Healthcare, 2007
Sigg,D.C., Iaizzo, P.A., Xiao,Y.F., He,B.: Cardiac Electrophysiology Methods and Models, Springer, 2010

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme BTB-PK Doctoral

    branch PK-BTB , 1 year of study, winter semester, elective specialised

  • Programme BTB-PP Doctoral

    branch PP-BTB , 1 year of study, winter semester, elective specialised

Type of course unit

 

Seminar

39 hod., optionally

Teacher / Lecturer

prof. MUDr. Marie Nováková, Ph.D.