Course detail
Modern methods in electrophysiology research
FEKT-DBT5Acad. year: 2018/2019
History and methods used in experimental cardiology. Isolated perfused Langendorff heart and its use for the isolation of cardiomyocytes. Methods for measuring of the membrane voltages and currents in different models. Electrocardiography and measuring the pressure in animal models. Registration of monophasic action potentials using various methods.
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Number of ECTS credits
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Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Course curriculum
2. Methods of experimental cardiology - overview.
3. Isolated perfused Langendorff heart.
4. Cardiomyocytes.
5. Voltage clamp.
6. Patch clamp.
7. Electrocardiography of animal models.
8. The acquisition of monophasic action potentials via different methods.
9. The acquisition of pressure on isolated heart and the heart in situ.
10. Biochemical methods used in experimental cardiology.
11. Genetic and genomic studies.
Work placements
Aims
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Sigg,D.C., Iaizzo, P.A., Xiao,Y.F., He,B.: Cardiac Electrophysiology Methods and Models, Springer, 2010
Recommended reading
Classification of course in study plans