Course detail
Electronics and Biosensors
FEKT-MPA-BSNAcad. year: 2021/2022
The concept of the course is focused on the fundamental aspects of biosensors and electronic biochips. Students will gain an overview of biosensors, especially of electrochemical, optical, piezoelectric and calorimetric type. They will get knowlegde about biocatalytic (enzymes) and bioaffinity (antigen-antibody, DNA or biomimetic) biosensors and their application in medicine, environment and food area. A part of the course will be devoted to the sensor technology (lithography, miniaturization, microfluidics) and nanomaterial (nanoparticles, nanowires) application in biosensing.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Department
Learning outcomes of the course unit
Prerequisites
Co-requisites
Planned learning activities and teaching methods
Assesment methods and criteria linked to learning outcomes
Lectures: oral exam
Practical course: protocols and test
Course curriculum
1. Introduction and history of biosensors, classification, applications. Characteristics of biosensors.
2. Introduction to electronics.
3. Electrochemical biosensors - introduction, types of electrodes, two- and multi-electrode circuits. Ampereometric measurement of oxygen, hydrogen peroxide and NADH, oxidase and dehydrogenase based biosensors.
4. Mediators of electron transfer. Potentiometric biosensors, ISFET, LAPS, voltammetric techniques.
5. Conductometric biosensors and impedance measurement.
6. Calorimetric biosensors and piezoelectric biosensors.
7. Optical biosensors - direct and indirect measurement principles; luminescence, optical fibers, SPR technology.
8. Enzyme biosensors, whole cell biosensors.
9. Affinity biosensors - immunosensors and nucleic acid biosensors.
10. Methods of biomolecule immobilization - physical and chemical immobilization.
11. Nanoparticles and nanostructured surfaces in biosensors.
12. Production and miniaturization of sensors. Screen printing, lithography, biochips.
13. Fundamentals of microfluidics, lab-on-chip, commercial biosensors.
Practical course:
1. Impedance measurement of bioaffinity interaction
2. Conductivity and pH measurement
3. Measurement of oxygen
4. Electrochemical measurement of heavy metal in solutions
5. Electrochemical measurement of glucose and ascorbic acid using carbon electrode
6. Amperometric enzymatic biosensor for dection of glucose
7. Credit test
Work placements
Aims
Students will understand the basics of biorecognition events (bioaffinity, biocatalytic) and get knowledge in biosensor technologies (lithography, miniaturization, microfluidics), immobilization procedures and application of nanomaterials (nanoparticles, nanowires) in biosensing.
Specification of controlled education, way of implementation and compensation for absences
Recommended optional programme components
Prerequisites and corequisites
Basic literature
Jeong-Yeol Yoon: Introduction to Biosensors: From Electric Circuits to Immunosensors (EN)
Recommended reading
Classification of course in study plans