Czech

Not applicable.

Increasing the knowledge, skills and competencies of students is reflected in the following areas:
1. Students will learn how to practically implement ecotoxicological bioassays and interpret the obtained results. This means that they will be able to design, organise realisation of tests and to interpret results of ecotoxicological evaluation of different types of matrices (chemicals, wastes, contaminated, environmental matrices) in accordance with requirements of current legislation.
2. Students will be able to select optimal ecotoxicity tests (aquatic/contact) ant to design optimal battery of ecotoxicity tests (standard or alternative) in accordance with the objectives of the concrete study;
3. On the base of results of ecotoxicological tests (LC50, EC50 etc.) students will be able to predict the influence of various chemicals and contaminated environmental matrices on biota of the specific ecosystem.
4. They will be able to confront all findings with the requirements of current legislation of the Czech Republic and the EU on the evaluation of chemicals and waste with regard to environmental protection.
All findings will be confronted with the requirements of current legislation of the Czech Republic and the EU concerning process assessment of chemicals and wastes with regard to environmental protection.

Basic knowledge of toxicology, biology, ecology, environmental chemistry, fundamentals of statistic data evaluation. Practical skills necessary to work in a chemical laboratory obtained in basic laboratory classes.

Not applicable.

The course uses teaching methods in form of Laboratory exercise - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

The basis for obtaining the credit is:
1. developing protocols of the required quality from practical tasks
2. completing all laboratory tasks
3. final presentation of ecotoxicity test results.

1. Basic principles and reasons for testing of chemicals and wastes. Legislation in the field of ecotoxicological evaluation of chemicals, sewage sludge and sediments. Importance and the main principles of aquatic and terrestrial ecotoxicology, Bioassays - definition, importance, principles, classification. Preparation of chemicals for ecotoxicological testing, concentration range, limit, preliminary and final tests. Waste water leachates preparation.
2. Standard tests (ISO, OECD) versus alternative tests. The using alternative tests in aquatic ecotoxicology - Thamnotoxkit FTM, Daphtoxkit FTM - practical implementation.
3. Standard tests in aquatic arrangement - tests on Lemna minor, Sinapis alba – practical implementation.
4. Test on algae Desmodesmus subspicatus, on bacteria Vibrio fischeri and on fish Brachydanio rerio – presentation.
5. Tests in contact arrangement – sediment testing on ostracoda Heterocypris incongruens (Ostracodtoxkit).
6. Tests in contact arrangement - screening tests on seeds of salad Lactuca sativa - practical implementation.
7. Artificial soil preparation for terrestrial tests, pH and humidity adjustment, concentration series in contact arrangement of tested matrices preparation.
8. Test on snail Helix aspersa aspersa.
9. Acute, chronic tests on earthworm Eisenia fetida, Test of escape behavior on Eisenia fetida – presentation.
10. Test on organism Folsomia candida, escape behavior test.
11. Test on organism Vibrio fischeri.
12. Calculation of ecotoxicological values (LC, EC, IC etc.) and their presentation with regards of present legislation in field chemicals and waste and prediction of their effects on ecosystem.
13. Credit

Not applicable.

Gaining knowledge enabling the use of bioassays for ecotoxicological assessment of the level of contamination of the environment; familiarize students with the principles of ecotoxicological testing using standard and alternative ecotoxicity tests to evaluate the effects of various contaminated environmental matrices (waste water, soil, sediment) and chemicals and wastes on the live components of the ecosystem. Apply their knowledge in real situation - the choice of relevant test (tests battery) and proper arrangement of tests (in contact arrangement /aquatic arrangement of the tests) for predicting the effects on the different functional levels (producers, consumers, decomposers) ecosystems (aquatic / terrestrial), calculate the output ecotoxicological values (LC, EC, IC, NOEC, LOEC) and on their basis to estimate risk for the ecosystem.

Attendance at the laboratory exercise is compulsory, submission of the protocols, processing presentation of the results of ecotoxicological tests. 100% participation at exercise.

Not applicable.

Fargašová A.: Ekotoxikologické biotesty. Bratislava, Perfekt. 2009 (CS)
Fargašová A.: Environmentálna toxikológia a všeobecná ekotoxikológia. Bratislava -Orman, 2008 (CS)
Svobodová Z. a kol.: Ekotoxikologie, praktická cvičení. VFU Brno, 2010 (CS)

Not applicable.