Course detail

Advanced Light Microscopy - Imaging Theory

FSI-9MIAAcad. year: 2021/2022

The course explains principles of light microscopy of three-dimensional objects in terms of wave optics. Image properties are described using the formalism of 3D pupil and transfer functions. Properties of advanced microscopic techniques for 3D imaging are explained, especially those of confocal microscopy and coherence-controlled holographic microscopy.

Language of instruction

Czech

Mode of study

Not applicable.

Guarantor

prof. RNDr. Radim Chmelík, Ph.D.

Department

Institute of Physical Engineering (ÚFI)

Learning outcomes of the course unit

Knowledge of fundamentals of theoretical description of microscope imaging of 3D objects of their relation to experiment and competence to apply them in practice.

Prerequisites

Wave and Fourier optics, geometrical optics, fundamentals of light microscopy.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

The course is taught through lectures explaining the basic principles and theory of the discipline. The lecture is completed by advanced optical demonstrations and experiments with light microscopes.

Assesment methods and criteria linked to learning outcomes

Examination is oral. Detailed knowledge of explained matter is proved. Examined student can use a literature.

Course curriculum

Not applicable.

Work placements

Not applicable.

Aims

Understanding of principles of 3D light microscopy and microscopic imaging.
Acquiring of methods for fundamental 3D imaging characteristics calculation.

Specification of controlled education, way of implementation and compensation for absences

Presence at lectures is not obligatory, but recommended.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Min Gu: Principles of Three-Dimensional Imaging in Confocal Microscopes (EN)
Radim Chmelik et al.: The Role of Coherence in Image Formation in Holographic Microscopy, Progress in Optics 59 (2014) 267-335. (EN)
Radim Chmelík.: Trojrozměrné zobrazení v mikroskopii. FSI VUT v Brně, Brno 2003 (CS)
Tony Wilson: Confocal Microscopy. Academic Press, London 1990 (EN)

Recommended reading

J. W. Goodman: Introduction to Fourier Optics. 2nd ed.. McGraw-Hill, New York 1996 (EN)
M. Born, E. Wolf: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999 (EN)

Classification of course in study plans

  • Programme D-FIN-P Doctoral 1 year of study, summer semester, recommended course
  • Programme D-FIN-K Doctoral 1 year of study, summer semester, recommended course

Type of course unit

 

Lecture

20 hod., optionally

Teacher / Lecturer

prof. RNDr. Radim Chmelík, Ph.D.

Syllabus

1. Important knowledge in the field of light microscopy and wave optics
2. Light wave interaction with an object
3. Imaging from the point of view of wave optics
4. Imaging by confocal micorscope
5. Imaging by interference and holographic microscope
6. Imaging by fluorescence microscope
7. Imaging in bright field
8. Another specific cases of imaging