Course detail

Systems Biology

FEKT-MPA-SYSAcad. year: 2021/2022

The course is oriented to gain knowledge of methods used in systems biology, creating models of cellular organisms and possibilities of their usage. It aims on computational methods used to describe behavior of living organisms on molecular level that are utilizable in cellular biology, biochemistry, and biotechnology.
Studied models are represented by extensive network graphs. Special attention is paid to both methodologies of model analysis, static as well as dynamic, especially using quantitative ODE models. The concept of hierarchy is followed and all functional layers, from gene regulatory network to signaling pathways and metabolic networks, are presented. Examples of models are given on systems of particular, especially unicellular, organisms.

Language of instruction

English

Number of ECTS credits

Mode of study

Not applicable.

Guarantor

doc. Mgr. Ing. Karel Sedlář, Ph.D.

Department

Department of Biomedical Engineering (UBMI)

Learning outcomes of the course unit

Students will be able to:
- mathematically describe the main components of gene expression
- mathematically describe the main components of signal transduction pathways
- analyze network graphs using network motifs
- name the main network motifs of transcriptional networks and signal-transduction pathways
- explain function of the main motifs of transcriptional networks and signal-transduction pathways
- describe experimental mathods in systems biology

Prerequisites

Students enrolled in this subject should be able to describe cellular systems, its main components regarding structure and function; analyze systems of ordinary differential equations and apply basic knowledge of probability distribution and combinatorics. In general, knowledge on the Bachelor's degree level is requested.

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Techning methods include lectures and computer laboratories. Course is taking advantage of e-learning (Moodle) system.

Assesment methods and criteria linked to learning outcomes

up to 16 points for reports from computer exercises
up to 14 points for completion and presentation of the semestral project
up to 70 points for exam.
Examination has an oral form.

Course curriculum

1. Introduction to systems biology
2. Laboratory techniques for systems biology
3. Model organisms, biological networks and pathways
4. Basics of dynamic analysis of continuous models
5. Enzyme kinetics
6. Regulation of transcription
7. Prediction of network motifs, autoregulation motif
8. FFL motif
9. Additional network motifs
10. Gene ontology
11. Qualitative Boolean models
12. Gene regulatory network inference and parameter estimation
13. Project presentations

Work placements

Not applicable.

Aims

The aim of the subject is to provide students with basic knowledge of computational models in cellular biology and way of their use, knowledge of analysis methods applied to models in systems biology.

Specification of controlled education, way of implementation and compensation for absences

Laboratory tutorials are compulsory, properly justified absence can be compensated based on agreement of the tutor (usually in the last semester week).

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Alon, U: An Introduction to Systems Biology, Design Principles of Biological Circuits. CRC, 2007, ISBN: 1-58488-642-0 (EN)
Dubitzky, W., Wolkenhauer, O., Cho, K.-H., Yokota, H., Encyclopedia of systems biology. Springer, New York 2013. ISBN 978-144-1998-644. (EN)
Konopka, A.K. Systems Biology: Principles, Methods, and Concepts. CRC, 2006, ISBN: 978-0824725204 (EN)
Maly, Ivan V. Systems biology. Humana Press, New York 2009. ISBN 978-1-934115-64-0. (EN)
Rosypal, S. Nový přehled biologie. Scientia, Praha 2003. ISBN 80-7183-268-5 (CS)

Type of course unit

Lecture

26 hod., optionally

Teacher / Lecturer

doc. Mgr. Ing. Karel Sedlář, Ph.D.

Syllabus

1. Introduction to systems biology - from molecular biology of cell to computational models
2. Modeling of biochemical systems - mathematical and computational models to describe processes in living organisms
3. Specific biochemical systems - mathematical modelling of biological and chemical processes in examples
4. Model fitting - design and verification of correct models, comparison to real living systems
5. Analysis of high-throughput data - recent methods used in bioinfnormatics and their implications to systems biology
6. Gene expression models - mathematical modelling of gene expression
7. Stochastic systems and variability - from deterministic to stochastic description of nearly chaotic biochemical processes
8. Network structures, dynamics, and function - networks of models and their use
9. Optimality and evolution - extended dynamic and adaptive models for evolving processes
10. Experimental techniques in molecular biology
11. Linear control systems in modelling
12. Computer modeling tools in practice
13. Systems biology for future

Exercise in computer lab

26 hod., compulsory

Teacher / Lecturer

Ing. Kateřina Šabatová
Ing. Jana Musilová, Ph.D.
Ing. et Ing. Jana Schwarzerová, MSc

Syllabus

1. Specific biochemical systems - mathematical modelling of biological and chemical processes in examples
2. Gene expression models - mathematical modelling of gene expression
3. Stochastic systems and variability - from deterministic to stochastic description of nearly chaotic biochemical processes
4. Optimality and evolution - extended dynamic and adaptive models for evolving processes
5. Selected computer modeling tools
6. Individual projects

Elearning

eLearning: currently opened course

VUT

Faculties

University Institutes

Parts

Systems Biology

Type of course unit