Course detail

Advanced fluorescence techniques

FCH-DC_PFTAcad. year: 2022/2023

Not applicable.

Language of instruction

Czech

Mode of study

Not applicable.

Learning outcomes of the course unit

Graduates of this course will understand the basic concepts of fluorescence spectroscopy. They will orientate themselves in stationary, time resolved and microscopic techniques. They will be provided with practical information on how to measure and analyze relevant data.

Prerequisites

physics, physical chemistry

Co-requisites

Not applicable.

Planned learning activities and teaching methods

Not applicable.

Assesment methods and criteria linked to learning outcomes

Exam: written and oral.

Course curriculum

- What is light and matter? Historical overview. Description tools.
- Scattering and absorption of radiation - electron, vibrational and rotational transitions. The fate of the excited state. Kinetics. Nonlinear optical phenomena.
- Solvatochromism and other environmental effects.
- Absorption spectrophotometry - uv-vis-ir, spectrophotometers and advanced techniques.
- Fluorescence spectroscopy stationary - fluorimeters, types of spectra, quantum yield
- Time-resolved fluorescence spectroscopy - time vs. time frequency domain, instrumentation and principles
- Microscopy - visible, fluorescent, confocal - principles and instrumentation
- Fluorescence correlation spectroscopy - autocorrelation function, cross-correlation function, instrumentation, measurement principles, models
- "Supermicroscopy" - STED, dSTORM, PALM, PAINT etc. - principles and applications
- The most common mistakes and errors in measurement.

Work placements

Not applicable.

Aims

Learning outcomes of the course unit The aim of the course is to present basic and advanced information about techniques using the fluorescence phenomenon.

Specification of controlled education, way of implementation and compensation for absences

Attendance to lectures is not required but is recommended.

Recommended optional programme components

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Lakowicz, J.R. Principles of Fluorescence 3rd ed., Springer, Baltimore, 2006 (EN)
Valeur, B.; Berberan-Santos, M.N. Molecular Fluorescence: Principles and Applications, Wiley‐VCH Verlag GmbH & Co. KGaA, 2012 (EN)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme DPCP_BCH Doctoral 1 year of study, winter semester, compulsory-optional
  • Programme DKCP_BCH Doctoral 1 year of study, winter semester, compulsory-optional