Czech

Not applicable.

Practical knowledges of some basis experimental techniques used in DNA research.

It is recomended to pass out the lecture Bioanalytical methods.

Not applicable.

The teaching is realised in 4 blocks. Each block runs 6 hours every 2 weeks. Part of the teaching is one introductery and one concluding hour.
The teaching requires 26 hours alltogether.
E-learning system LMS Moodle is available for teachers and students.

Laboratory course is finished by test and cotrol of protocols.

1. Safety of the work in laboratory. Arrangement of PCR laboratory. Pipetting of small volumes.
2. Cultivation of Lactobacillus cells for isolation of bacterial DNA. Estimation of purity of bacterial culture.
Cell lysis and preparing of crude cell lysate. Removing of proteins using phenol extraction. Precipitation of DNA with ethanol. Determination of DNA integrity using agarose gel electrophoresis. Preparation of a protocol in the form of poster.
3. Spectrophotometric estimation of DNA concentration and purity. Preparation of PCR mixture for amplification of specific part of Lactobacillus DNA using purified DNA. Preparation of negative control. PCR amplification. Detection of PCR product using agarose gel electrophoresis. Estimation of length of amplicon with help of DNA standard and programme Bionumerics. Preparation of a protocol in the form of poster.
4. Isolation of whole DNA from crude cell lysates of dairy product (probiotic drink) using magnetic particles P(HEMA-co-GMA). Amplification of DNA by PCR in real time. Preparation of a protocol in the form of poster.
5. Introduction to bioinformatics - lecture a practical excersize on PC.
6. Test and control of protocols.

Not applicable.

The aim of the laboratory course is to cover methodologies used in the work with DNA particularly its amplification in PCR. The next aim covers the preparation of poster.

Attendance in laboratory course is obligatory.

Not applicable.

Not applicable.

Španová A., Rittich B.: Analýza vybraných druhů baktérií mléčného kvašení pomocí metod molekulární biologie (CS)

Němcová, A.: Praktikum z molekulární biotechnologie, FCH VUT (elektronická učební podpora, dostupné na: https://moodle.vut.cz/course/view.php?id=242687) (CS)