Course detail
Technology in Cellular Engineering
FEKT-MPC-TBIAcad. year: 2022/2023
The course "Technology in Cell Engineering" introduces students to methods, technologies and instrumentation used in the fields of cell and tissue engineering in laboratories and research.
Language of instruction
Czech
Number of ECTS credits
5
Mode of study
Not applicable.
Guarantor
Learning outcomes of the course unit
Requirements for successful completion of the subject are specified by guarantor’s regulation updated for every academic year.
Prerequisites
Knowledge of biology, mathematics and physics accordant with the subjects of previous bachelor’s study is expected.
Co-requisites
Not applicable.
Planned learning activities and teaching methods
Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.
Assesment methods and criteria linked to learning outcomes
Teaching methods depend on the type of course unit as specified in the article 7 of BUT Rules for Studies and Examinations.
Course curriculum
1. Cultivation and preparation of cells and tissue, automation of processes in the cell laboratory.
2. Technology for cell counting and sorting, automated laboratory analyzers for tissue engineering.
3. Mechanical properties and methods of their measurement. Holographic microscopy and its applications.
4. Microscopic techniques, light field observation, contrast techniques, fluorescence microscopy.
5. Special microscopic imaging techniques in cell engineering. Recording techniques for microscopy.
6. Electron microscopy in cell engineering, correlation microscopy, preparation of biological samples for electron microscopy.
7. Methods for measuring electrical activity of excitable tissue using microelectrode arrays and microelectrode systems.
8. Methods and technologies for cell migration analysis.
9. Nanotechnology in regenerative and cancer medicine.
10. 3D bioprinting and its application in regenerative medicine.
11. Technologies and methods used in optogenetics.
12. Microfluidic systems and their use in cell engineering.
2. Technology for cell counting and sorting, automated laboratory analyzers for tissue engineering.
3. Mechanical properties and methods of their measurement. Holographic microscopy and its applications.
4. Microscopic techniques, light field observation, contrast techniques, fluorescence microscopy.
5. Special microscopic imaging techniques in cell engineering. Recording techniques for microscopy.
6. Electron microscopy in cell engineering, correlation microscopy, preparation of biological samples for electron microscopy.
7. Methods for measuring electrical activity of excitable tissue using microelectrode arrays and microelectrode systems.
8. Methods and technologies for cell migration analysis.
9. Nanotechnology in regenerative and cancer medicine.
10. 3D bioprinting and its application in regenerative medicine.
11. Technologies and methods used in optogenetics.
12. Microfluidic systems and their use in cell engineering.
Work placements
Not applicable.
Aims
The aim of the course is to provide students with knowledge of the technologies and methods used in cell and tissue engineering laboratories.
Specification of controlled education, way of implementation and compensation for absences
Extent and forms are specified by guarantor’s regulation updated for every academic year.
Recommended optional programme components
Not applicable.
Prerequisites and corequisites
Not applicable.
Basic literature
Alberts B., Johnson A., Walter P.: Molecular Biology of the Cell. (CS)
Ankur Singh, Akhilesh K. Gaharwar: Microscale Technologies for Cell Engineering (CS)
Bengt Nölting: Methods in Modern Biophysics (CS)
José Luis R. Arrondo, Alicia Alonso: Advanced Techniques in Biophysics. (CS)
J.Šimurda: Bioelektrické jevy, elektronická skripta (CS)
Ankur Singh, Akhilesh K. Gaharwar: Microscale Technologies for Cell Engineering (CS)
Bengt Nölting: Methods in Modern Biophysics (CS)
José Luis R. Arrondo, Alicia Alonso: Advanced Techniques in Biophysics. (CS)
J.Šimurda: Bioelektrické jevy, elektronická skripta (CS)
Recommended reading
Not applicable.
Classification of course in study plans
- Programme MPC-BTB Master's 0 year of study, summer semester, elective