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FCH-MC_PFSAcad. year: 2023/2024
Learning outcomes of the course unit The aim of the course is to introduce students to various fluorescence techniques and their application with interpretation of obtained results. Students will be introduced to the phenomenon of fluorescence from the basis of light-matter interaction to super-differentiated microscopic techniques. The following techniques will be discussed: time-resolved emission spectroscopy, anisotropy, resonance energy transfer, fluorescence quenching, fluorescence correlation spectroscopy, two-color fluorescence cross-correlation spectroscopy. Practical examples, their advantages, limitations and methods of interpretation will be presented for each method.
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