Course detail

Biochemistry I

FCH-BA_BCH1Acad. year: 2023/2024

Organization and composition of living cells and cellular compartments. Amino acids - composition and structure, peptides, proteins. Methods of isolation and separation of proteins. Functional roles of proteins. Nucleic acids. Carbohydrates. Lipids. Biomembranes. Metabolic pathways. termodynamic relationships and energy-rich components. Enzymology, enzyme catalysis. Enzyme kinetics, inhibition of enzymes. Regulation of enzyme activity. Nucleic acids. Applied biochemistry.

Language of instruction

English

Number of ECTS credits

4

Mode of study

Not applicable.

Guarantor

prof. RNDr. Ivana Márová, CSc.

Department

Institute of Food Science and Biotechnology (ÚCHPBT)

Offered to foreign students

Of all faculties

Entry knowledge

Knowledge of organic chemistry, basic knowledge of physical chemistry.

Rules for evaluation and completion of the course

Credit test. Examination - written and oral part.
none

Aims

The aim of the course Biochemistry I is to introduce cell composition and to characterize structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, structure of biomemrbanes and types of membrane transport are explained and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, methods of isolation and characterization of biopolymers are introduced and some applications including enzyme engineering and genetic engineering are discussed.
After passing of the course Biochemistry I is student mastered of overview about cell composition, structure, properties and function of basic groups of biomolecules, e.g. amino acids, proteins, saccharides, lipids and nucleic acids. Further, basic knowledge about structure of biomemrbanes and types of membrane transport are introduced and basic principles of enzyme catalysis, kinetics and regulation of enzyme activity are elucidated. Next, overview about methods of isolation and characterization of biopolymers will be obtained and some applications including enzyme engineering and genetic engineering will be discussed.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Metzler D.A.: Biochemistry. Academic Press, 2003. (EN)

Recommended reading

Berg, Tymoczko, Stryer: Biochemistry. W.H.Freeman, 2002. (EN)
Alberts et al: Molecular Biology of the Cell. Garland Science, 2003. (EN)

Elearning

eLearning: currently opened course

Classification of course in study plans

  • Programme BKCP_CHCHTE Bachelor's 2 year of study, summer semester, compulsory
  • Programme BKCP_AAEFCH Bachelor's 2 year of study, summer semester, compulsory
  • Programme BPCP_AAEFCH Bachelor's 2 year of study, summer semester, compulsory
  • Programme BPCP_CHCHTE Bachelor's 2 year of study, summer semester, compulsory
  • Programme BKCP_CHTM Bachelor's 2 year of study, summer semester, compulsory-optional
  • Programme BPCP_CHTM Bachelor's 2 year of study, summer semester, compulsory-optional

  • Programme BPCP_CHTPO Bachelor's

    specialization CHPL , 2 year of study, summer semester, compulsory
    specialization PCH , 2 year of study, summer semester, compulsory
    specialization BT , 2 year of study, summer semester, compulsory

  • Programme BKCP_CHTPO Bachelor's

    specialization PCH , 2 year of study, summer semester, compulsory
    specialization BT , 2 year of study, summer semester, compulsory
    specialization CHPL , 2 year of study, summer semester, compulsory

  • Programme BPCP_CHMA Bachelor's 2 year of study, summer semester, compulsory
  • Programme NPAP_ENVI Master's 1 year of study, summer semester, compulsory
  • Programme BPCP_CHTN Bachelor's 2 year of study, summer semester, compulsory

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

Ing. Simona Dzurendová, PhD
prof. RNDr. Ivana Márová, CSc.

Syllabus

Organization and composition of living cells and cellular compartments.
Amino acids - composition and structure, peptides, proteins.
Methods of isolation, purification and separation of proteins.
Functional roles of proteins.
Carbohydrates - monosaccharides, polysaccharides, glycoproteins.
Lipids - structure and classification. Triglycerides, phospholipids. Isoprenoids.
Biomembranes. Membrane transport.
Enzymology, enzyme catalysis. Substrate specificity. Cofactors. Regulation of enzyme activity.
Enzyme kinetics, Michaelis-Menten equation. Inhibition of enzymes.
Metabolic pathways. termodynamic relationships and energy-rich components.
Nucleic acids - structure and function. DNA, RNA.
Applide biochemistry - modern biotechnology, genetic engineering.

Exercise

13 hod., compulsory

Teacher / Lecturer

prof. RNDr. Ivana Márová, CSc.

Syllabus

1. Amino acids and proteins - structure and distribution of amino acids, spatial structure of proteins, non-covalent bonds and their biochemical significance.
2. Carbohydrates - monosaccharides, polysaccharides, complex carbohydrates (glycoproteins, glycolipids).
3. Lipids and fatty acids - structure, simple and complex lipids, phospholipids, isoprenoids.
4. Biomembranes - structure and function, membrane transport.
5. Enzymes and biocatalysis. Substrate specificity. Brief nomenclature of enzymes. Cofactors. Enzyme kinetics and inhibition.
6. Nucleic acids and nucleotides - types, structure of nucleotides and nucleic acids, function in the organism, genetic code.
7. Carbohydrate metabolism - glycolysis, fermentation and their industrial use.
8. Tricarboxylic acid cycle. Gluconeogenesis. Glyoxalate cycle.
9. Biological oxidation. Electron transport and oxidative phosphorylation. Macroergic compounds. Photosynthesis.
10. Lipid metabolism - beta-oxidation of fatty acids, fatty acid biosynthesis.
11. Amino acid catabolism - urea cycle, transformations of the carbon skeleton of amino acids. Biosynthesis of amino acids.
12. Biosynthesis of nucleic acids - replication. 13. Process of gene expression - transcription, translation. Proteosynthesis.

Elearning

eLearning: currently opened course