Course detail
Biochemistry
FEKT-BPC-BCHAcad. year: 2023/2024
The subject deals with the fundamental concepts of general and physical chemistry, structures and reactions of biochemical organic compounds and the basic metabolism in the eucaryonic cell. The subject also focuses on the biochemistry and pathobiochemistry of organs, on the role of clinical biochemistry in detecting metabolic disorders and on the principles of clinical biochemical methods.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Department
Entry knowledge
Rules for evaluation and completion of the course
Seminars, laboratory exercises and excursions are mandatory. They can be replaced in consultation with the teacher.
Aims
The course participant is able to:
Explain the importance of basic physico-chemical terms.
Explain the importance of micro- and macro-biogenic elements in living organisms.
Calculate the concentration, pH and osmotic pressure of the solutions.
Describe the structure of organic compounds important for metabolism.
Explain the importance of metabolism of living organisms.
Describe the basic metabolic processes at the cellular and organ level.
Describe the basic principles of clinical and biochemical methodologies and discuss their use.
Study aids
Prerequisites and corequisites
Basic literature
Studijní materiály uložené v interaktivní osnově předmětu (IS MU) (CS)
Recommended reading
Classification of course in study plans
- Programme BPC-BTB Bachelor's 1 year of study, winter semester, compulsory
Type of course unit
Lecture
Teacher / Lecturer
Syllabus
Lipides, sacharides.
Proteins, nucleus acids, vitamins.
Enzymes and cofactors.
General principles of metabolism. Macroergonic compounds. The citrate cycle. The respiratory system.
Metabolism of lipides, sacharides and its disorders.
Metabolism of proteins and aminoacids and its disorders.
The principles of metabolism regulation.
Methods of clinical biochemistry.
Biochemistry of bodily fluids.
Biochmistry of kidneys, liver.
Fundamentals seminar
Teacher / Lecturer
Syllabus
Oxidation-reduction reactions. Solubility of salts, caculation from the solubility product
An outline of the methods used in clinical biochemistry (optical methods, chromatographic and electrophoretic methods, immunochemical methods). Biochemical analyzers.
Factors affecting the result and interpretation of clinical-biochemical examination. Enzymes in clinical diagnostics.
Examples of clinical-biochemical data evaluation in diagnostics.
Laboratory exercise
Teacher / Lecturer
Syllabus
Measurement and calculation of pH solutions of acids, bases and salts, hydrolysis, pH-meters
Fluid chromatography - types and basic techniques, gas chromatography
Spectrophotometry
Excursion to a clinical-biochemical laboratory
Examination of the metabolism of lipides and cholesterol (Reflotron, Akutrend, photometry)
Examination of proteins in blood serum (determination of albumin, electrophoresis, ELISA, latex-agl, test)
Analysis of urine (pathological components, drugs, microalbuminurite, SDS/elfo proteins)
Field trip
Teacher / Lecturer
Syllabus