Course detail

Electronics and Biosensors

FEKT-MPA-BSNAcad. year: 2023/2024

The concept of the course is focused on the fundamental aspects of biosensors and electronic biochips. Students will gain an overview of biosensors, especially of electrochemical, optical, piezoelectric and calorimetric type. They will get knowlegde about biocatalytic (enzymes) and bioaffinity (antigen-antibody, DNA or biomimetic) biosensors and their application in medicine, environment and food area. A part of the course will be devoted to the sensor technology (lithography, miniaturization, microfluidics) and nanomaterial (nanoparticles, nanowires) application in biosensing.

Language of instruction

English

Number of ECTS credits

5

Mode of study

Not applicable.

Guarantor

doc. Mgr. Zdenka Fohlerová, Ph.D.

Department

Department of Microelectronics (UMEL)

Entry knowledge

The basic knowledge of biochemistry and electrochemistry are required.

Rules for evaluation and completion of the course

Lectures: oral exam
Practical course: protocols and test
Specification of the course and its implementation is specified by guarantor’s regulation updated for every academic year.

Aims

The objective of course is to provide an overview of biosensors including the physico-chemical theory, construction and application in clinical analysis, environment, food and safety areas.
Students will understand the basics of biorecognition events (bioaffinity, biocatalytic) and get knowledge in biosensor technologies (lithography, miniaturization, microfluidics), immobilization procedures and application of nanomaterials (nanoparticles, nanowires) in biosensing.
Students will understand the biosensors: their fundamentals, designs, types and most recent impactful applications.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Biosensors and Biodetection: Methods and Protocols, ISBN 978-1-4939-6911-1 (EN)
Jeong-Yeol Yoon: Introduction to Biosensors: From Electric Circuits to Immunosensors (EN)

Recommended reading

Not applicable.

Elearning

eLearning: currently opened course

Classification of course in study plans

  • Programme MPAD-MEL Master's 0 year of study, winter semester, elective
  • Programme MPA-MEL Master's 0 year of study, winter semester, elective

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

doc. Mgr. Zdenka Fohlerová, Ph.D.

Syllabus

1. Introduction and history of biosensors, distribution, use. Characteristics of biosensors.

2. Introduction to electronics I.

3. Introduction to electronics II.

4. Methods of immobilization of biomolecules.

5. Electrochemical biosensors – introduction, types of electrodes. Amperometric measurement of oxygen, hydrogen peroxide and NADH, oxidase and dehydrogenase.

6. Electron transfer mediators. Potentiometric biosensors, ISFET, LAPS, voltammetric techniques.

7. Conductometric biosensors and impedance measurement.

8. Calorimetric biosensors and piezoelectric biosensors.

9. Optical biosensors – direct and indirect measurement principles; optical fibers, reflectivetechniques etc.

10. Enzyme biosensors, cell biosensors. Principles and examples.

11. Affinity biosensors - immunosensors and hybridization of nucleic acids. Principles and examples.

12. Nanoparticles and nanostructured surfaces in biosensors.

13. Basics of sensor production and miniaturization. Screen printing, lithography, etc. Fundamentals of microfluidics, lab-on-chip, POCT, commercial biosensors. 

Laboratory exercise

26 hod., compulsory

Teacher / Lecturer

doc. Mgr. Zdenka Fohlerová, Ph.D.

Syllabus

1. Measurement of bioaffinity interactions using an impedance transducer

2. Measurement of specific conductivity and pH

3. Measuring of oxygen using Clark's oxygen electrode

4. Electrochemical determination of heavy metals in solutions

5. Electrochemical determination of ascorbic acid with a carbon electrode

6. Amperometric determination of glucose using an enzymatic biosensor

7. Determination of volatile substances in the air 

Elearning

eLearning: currently opened course