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FCH-DA_PFTAcad. year: 2023/2024
The course is designed as a practically focused seminar, which will enable students to acquaint themselves with advanced fluorescence techniques on a suitable model system. Theoretical and practical lessons will alternate within the seminar. The theoretical parts will focus on specific aspects of preparation, measurement and interpretation of the fluorescence techniques and will build on the self-study of the fundamental fundamentals of the given techniques. The practical part includes the preparation of the model system itself (associative colloid system), measurement and evaluation. Within the subject, the following techniques will be discussed: time-resolved emission spectroscopy, anisotropy, resonance energy transfer, fluorescence quenching, fluorescence correlation spectroscopy, two-color fluorescence cross-correlation spectroscopy. Together with the introductory lesson of these 6 themes with the theoretical and practical part will create the framework of the semester. At the end of the semester, the basics will be created to create a short monograph on the behavior of the selected system from the perspective of advanced fluorescence techniques.
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