39 hod., optionally

Definition of the molecular biology subject. From atoms and molecules to living cells. Nucleic acids, proteins, lipids, polysaccharides. Chemical nature of biomacromolecules, basic interaction types. Organized structures, membranes, organelles, prokaryotic and eukaryotic cells. (0-6 lessons)

Structure and interactions of nucleic acids. DNA double helix. Left-handed DNA, hairpins, triplexes, tetraplexes. DNA supercoiling. Replication, transcription, recombination. Chemical reactivity of nucleic acids, non-covalent interactions. DNA damage and repair, mutagenesis. Nucleic acids-processing enzymes. RNAs and their biological functions. (7-20 lessons)

Methods of the nucleic acids studies. Optical methods, electrophoresis, Southern and northern blotting, DNA sequencing, DNA hybridization, PCR, enzymatic and chemical probes of DNA structure, DNA footprinting. Recombinant DNA. Synthetic analogues of nucleic acids, DNA labeling. Biosensors, gene chips, genomics. Electrochemistry of nucleic acids, interactions with electrically charged surfaces. Aptamers. Nanotechnology in nucleic acids analysis. (21-28 lessons)

Proteins. Amino acids, primary, secondary, tertiary and quaternary structure of proteins. Conjugated proteins. Genetic code and proteosynthesis. Denaturation and aggregation of proteins. Protein engineering. Nucleic acids-protein interactions. (29-34 lessons)

Methods of protein studies. Optical methods, electrophoresis, western blotting, immunochemical techniques. Chemical probes, protein sequencing. Proteomics, 2D electrophoresis, mass spectrometry. Protein chips, immunosensors, enzyme sensors. Protein electrochemistry; conjugated proteins bearing reversible redox centers and non-conjugated proteins, catalytic hydrogen evolution on mercury and amalgam electrodes. Nanotechnology in protein analysis. (35-39 lessons)

3 hod., compulsory

Practical demonstrations of methods of the nucleic acids and protein analysis in laboratories of the Institute of Biophysics, ASCR, v.v.i.