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Course detail
FSI-TEB-AAcad. year: 2024/2025
Basics of biophotonics including principles of light microscopy, fluorescence, confocal microscopy, quantitative phase imaging especially in holographic microscopy, and polarization microscopy. Basics of cell biology in vitro: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopy observations. Protocols of in vitro investigations of cell behavior. The role of light microscopy techniques for the study of live cells in vitro:
Applications in neurosciences and cancer research.
Demonstrations and practical sections in the laboratory.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Department
Entry knowledge
Rules for evaluation and completion of the course
Student assessment will take into consideration his/her work at the laboratory and will depend on the result of the final discussion.Presence in all teaching units is required because of their mutual links. In the case of student absence, the teacher will specify alternative requirements.
Aims
The objective of this theme is the introduction of biophotonics including light microscopy techniques for biology. The theme also includes the basics of cell biology and the application of light microscopy in studies of live cells in vitro, and example applications in neurosciences and cancer research.Acquiring of widened ability to interdisciplinary professional activity.
Study aids
Prerequisites and corequisites
Basic literature
Recommended reading
Classification of course in study plans
Lecture
Teacher / Lecturer
Syllabus
Basic principles of light microscopy for biologyCell biology and preparation of samples for microscopyInterferometry and holographic microscopyFluorescence, confocal and 2-photon microscopyApplications in tumor and cell biologyPolarization microscopy and imaging of extracellular matrix
Laboratory exercise