Course detail

Advanced Light Microscopy - Imaging Theory

FSI-9MIAAcad. year: 2024/2025

The course explains principles of light microscopy of three-dimensional objects in terms of wave optics. Image properties are described using the formalism of 3D pupil and transfer functions. Properties of advanced microscopic techniques for 3D imaging are explained, especially those of confocal microscopy and coherence-controlled holographic microscopy.

Language of instruction

Czech

Mode of study

Not applicable.

Guarantor

prof. RNDr. Radim Chmelík, Ph.D.

Department

Institute of Physical Engineering (ÚFI)

Entry knowledge

Wave and Fourier optics, geometrical optics, fundamentals of light microscopy.

Rules for evaluation and completion of the course

Examination is oral. Detailed knowledge of explained matter is proved. Examined student can use a literature.
Presence at lectures is not obligatory, but recommended.

Aims

Understanding of principles of 3D light microscopy and microscopic imaging.
Acquiring of methods for fundamental 3D imaging characteristics calculation.
Knowledge of fundamentals of theoretical description of microscope imaging of 3D objects of their relation to experiment and competence to apply them in practice.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Min Gu: Principles of Three-Dimensional Imaging in Confocal Microscopes (EN)
Radim Chmelik et al.: The Role of Coherence in Image Formation in Holographic Microscopy, Progress in Optics 59 (2014) 267-335. (EN)
Radim Chmelík.: Trojrozměrné zobrazení v mikroskopii. FSI VUT v Brně, Brno 2003 (CS)
Tony Wilson: Confocal Microscopy. Academic Press, London 1990 (EN)

Recommended reading

J. W. Goodman: Introduction to Fourier Optics. 2nd ed.. McGraw-Hill, New York 1996 (EN)
M. Born, E. Wolf: Principles of Optics. 7th (expanded) ed.. Cambridge University Press, Cambridge 1999 (EN)

Classification of course in study plans

  • Programme D-FIN-P Doctoral 1 year of study, summer semester, recommended course
  • Programme D-FIN-K Doctoral 1 year of study, summer semester, recommended course

Type of course unit

 

Lecture

20 hod., optionally

Teacher / Lecturer

prof. RNDr. Radim Chmelík, Ph.D.

Syllabus

1. Important knowledge in the field of light microscopy and wave optics
2. Light wave interaction with an object
3. Imaging from the point of view of wave optics
4. Imaging by confocal micorscope
5. Imaging by interference and holographic microscope
6. Imaging by fluorescence microscope
7. Imaging in bright field
8. Another specific cases of imaging