Course detail
Concepts of Biofotonics
FSI-9VKBAcad. year: 2024/2025
The course provides an overview of modern techniques using electromagnetic radiation suitable for studying the structure, function and dynamics of biological macro and micro objects and of methods applicable to the detection, diagnosis and treatment of diseases in a non-invasive way.
Language of instruction
Czech
Mode of study
Not applicable.
Guarantor
Department
Entry knowledge
Knowledge of geometric, wave and physical optics.
Rules for evaluation and completion of the course
Doctoral exam in the presented subject extent examining in particular the understanding of the physical nature of biophotonics methods.
Attendance at the lecture is optional.
Attendance at the lecture is optional.
Aims
The aim is to understand the principles of application of photonics in specific areas of biology and medicine.
Knowledge of physical and optical principles of biophotonics, which gives a deeper basis for its practical use.
Knowledge of physical and optical principles of biophotonics, which gives a deeper basis for its practical use.
Study aids
Not applicable.
Prerequisites and corequisites
Not applicable.
Basic literature
J. B. Pawley: “Handbook of Biological Confocal Microscopy”, 2nd Edition, Plenum Press, 1995. (EN)
Krafft, C: Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs. J Biophotonics 9 (2016) 1362-1375. (EN)
P. N. Prasad: “Introduction to Biophotonics”, John Wiley & Sons, Inc., 2003. (EN)
Krafft, C: Modern trends in biophotonics for clinical diagnosis and therapy to solve unmet clinical needs. J Biophotonics 9 (2016) 1362-1375. (EN)
P. N. Prasad: “Introduction to Biophotonics”, John Wiley & Sons, Inc., 2003. (EN)
Recommended reading
Not applicable.
Classification of course in study plans
Type of course unit
Lecture
20 hod., optionally
Teacher / Lecturer
Syllabus
1. Recapitulation of electromagnetic radiation theory.
2. Sources of radiation, lasers.
3. Interaction of radiation with matter, effect of radiation on tissues.
4. Basic methods of optical microscopy.
5. Confocal microscopy, OCT / OCM, holographic microscopy.
6. Fluorescence microscopy, Raman microscopy.
7. Flow cytometry.
8. Optical manipulation with microobjects, application of lasers in medicine.
9. Photodynamic therapy, optical biosensors.
10. Application of micro- and nano-opto-technologies in medicine (retinal replacements).
2. Sources of radiation, lasers.
3. Interaction of radiation with matter, effect of radiation on tissues.
4. Basic methods of optical microscopy.
5. Confocal microscopy, OCT / OCM, holographic microscopy.
6. Fluorescence microscopy, Raman microscopy.
7. Flow cytometry.
8. Optical manipulation with microobjects, application of lasers in medicine.
9. Photodynamic therapy, optical biosensors.
10. Application of micro- and nano-opto-technologies in medicine (retinal replacements).