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FCH-BC_ZMAcad. year: 2024/2025
Students will be introduced to biotechnological processes used for production of selected microbial, mammalian, hybridome and plant substances. Further, principles of fermentation procedures, separation of cells from media, dissintegration processes, separation techniques and purification of biomolecules will be discussed. As a part of this course basic methods of recombinant DNA technology: isolation and characterization of DNA and RNA, restriction endonucleases and other enzymes, cloning, polymerase chain reaction, gene mapping, methods of transformation, transformation vectors - plasmids, phages, cosmids, YAC will be introduced. Methods of identification of recombinant DNA will be explained. Gene expression in microbial (bacteria, yeast) and animal expression systems will be described including expression markers. Identification and purification of recombinant proteins (PAGE-SDS, Western blot, immunodetection) will be introduced too.
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