Course detail
Technology in Cellular Engineering
FEKT-MPA-TBIAcad. year: 2024/2025
The subject "Technology in cell engineering" introduces students to the methods, technologies and instrumentation used in the fields of cell and tissue engineering in laboratories and research.
Language of instruction
Number of ECTS credits
Mode of study
Guarantor
Offered to foreign students
Entry knowledge
Rules for evaluation and completion of the course
The final grade for the course will be determined by the scores obtained from the midterm tests and the activity
exercises (max. 20 points in total) and the final written examination (max. 80 points).
Only students who have been awarded credit may take the final exam.
- obtaining at least 15 points in the laboratory exercises, including points for the continuous written tests,
- a non-zero score on the continuous written tests,
- a non-zero score on the protocols or homework assignments,
- meeting full attendance requirements.
More detailed conditions for successful completion of the course are set out in the annually updated decree of the course coordinator.
Aims
Study aids
Prerequisites and corequisites
Basic literature
Ankur Singh, Akhilesh K. Gaharwar: Microscale Technologies for Cell Engineering (CS)
Bengt Nölting: Methods in Modern Biophysics (CS)
José Luis R. Arrondo, Alicia Alonso: Advanced Techniques in Biophysics. (CS)
Recommended reading
Elearning
Classification of course in study plans
Type of course unit
Lecture
Teacher / Lecturer
Syllabus
2. Technology for cell counting and sorting, automated laboratory analyzers for tissue engineering.
3. Microscopic techniques, bright field observation, contrast techniques, fluorescence microscopy.
4. Special microscopic imaging techniques, DHM, AFM.
5. Electron microscopy in cell engineering, correlation microscopy, preparation of biological samples for electron microscopy.
6. Application of biomaterials in biomedical engineering.
7. 3D bioprinting and its application in regenerative medicine.
8. Nanotechnology in regenerative and cancer medicine.
9. Mechanical properties of cells and methods of their measurement.
10. Methods and technologies for cell migration analysis.
11. Technologies and methods used in optogenetics.
12. Technologies applied to animal models. Virtual reality in biomedical applications.
Exercise in computer lab
Teacher / Lecturer
Syllabus
2. TEER measurement of cultivated cells
3. Observation techniques, fluroescence microscopy
4. Cells segmentation (image analysis)
5. Cells migrations, data processing
6. Signals processing MEA+AFM
7. Fusion 360 basics
8. 3D printing in cardiology, segmentation (Slicer)
9. Biomaterials preparation
10. 3D bioprinting: practical task (Inkredible)
11. Virtual Reality for Fusion models
12. Excursion
Laboratory exercise
Teacher / Lecturer
Syllabus
2. TEER measurement of cultivated cells
3. Observation techniques, fluroescence microscopy
4. Cells segmentation (image analysis)
5. Cells migrations, data processing
6. Signals processing MEA+AFM
7. Fusion 360 basics
8. 3D printing in cardiology, segmentation (Slicer)
9. Biomaterials preparation
10. 3D bioprinting: practical task (Inkredible)
11. Virtual Reality for Fusion models
12. Excursion
Elearning