Course detail

Light Microscopy

CEITEC VUT-DS135AAcad. year: 2024/2025

The course explains the basic principles, practical procedures, and applications of light microscopy in the following points:

  1. Fundamentals of optics for light microscopy
  2. Concept of resolution
  3. Phase contrast and darkfield microscopy
  4. Polarization microscopy
  5. Differential Interference Contrast Microscopy and Modulation Contrast Microscopy
  6. Fluorescence Microscopy
  7. Confocal Laser Scanning and Two-Photon Excitation Fluorescence Microscopy
  8. Superresolution Imaging
  9. Imaging Living Cells with the Microscope
  10. Fundamentals of Digital Imaging and Image Processing

Language of instruction

English

Mode of study

Not applicable.

Guarantor

prof. RNDr. Radim Chmelík, Ph.D.

Department

Central European Institute of Technology BUT (CEITEC VUT)

Entry knowledge

Fundamentals of geometric and wave optics.

Rules for evaluation and completion of the course

Oral examination.

Aims

Not applicable.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Douglas B. Murphy, Michael W. Davidson (2013) Fundamentals of Light Microscopy and Electronic Imaging. Wiley‐Blackwell. DOI 10.1002/9781118382905 (EN)
https://micro.magnet.fsu.edu/primer/ (EN)
https://zeiss-campus.magnet.fsu.edu/articles/basics/index.html (EN)
James B. Pawley (2006) Handbook of Biological Confocal Microscopy. Springer. DOI 10.1007/978-0-387-45524-2 (EN)
Max Born, Emil Wolf (2020) Principles of Optics. Cambridge University Press. DOI 17/9781108769914 (EN)

Recommended reading

Not applicable.