Course detail

Advanced Light Microscopy - Imaging Theory

CEITEC VUT-DS142Acad. year: 2024/2025

The course explains the 3D scalar imaging theory for a holographic microscope with incoherent illumination source using the transfer function and point spread function. The basics of the theory are also applicable for other microscopies.

  1. The basic building blocks of the theory.
  2. Wave propagation through microscope arms and basic imaging formula.
  3. Application of the first Born approximation, coherent transfer function.
  4. Amplitude point spread function and its properties.
  5. Simplification for 2D samples.
  6. Imaging of objects in scattering media.
  7. Another applications of the coherence gate.

Language of instruction

Czech

Mode of study

Not applicable.

Entry knowledge

Wave and Fourier optics, geometric optics, fundamentals of light microscopy.

Rules for evaluation and completion of the course

The exam is oral with the use of literature.

Aims

Not applicable.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Min Gu: Principles of Three-Dimensional Imaging in Confocal Microscopes (EN) (CS)
Chmelík et al. The Role of Coherence in Image Formation in Holographic Microscopy. Progress in Optics (Edited by Emil Wolf), Volume 59, Pages 267-335 (2014). (EN)
Tony Wilson: Confocal Microscopy. Academic Press, London 1990 (EN) (CS)

Recommended reading

Not applicable.