Over the past several decades, different materials have been explored for medical applications. To screen biocompatible materials, it is necessary to evaluate them in terms of in vitro cell responses, cytocompatibility, and cytotoxicity. The aim of this course is to teach theoretical foundations of cell culture techniques to determine hemocompatibility and biocompatibility of biomaterials in vitro. The students will acquire an overview of currently applications of biomaterials, they will learn to discern among the cell types, culture techniques, and the parameters and methods to evaluate. The course seeks to promote the student's critical posture towards materials used in medicine and apply theoretical concepts in solving their research inquiries.