Course detail

Experimental biophotonics

CEITEC VUT-DS103AAcad. year: 2024/2025

Principles of light microscopy, fluorescence, confocal microscopy, quantitative phase imaging in holographic microscopy, and polarisation microscopy. Basics of cell biology: structure and functions of the cell; isolation of cells from multicellular organisms and their maintenance in vitro; mastering the specialty of preparation of live cells for microscopical observations. Protocols of in vitro investigations of cell behavior. The role of light microscopy techniques for the study of live cells in vitro:

Applications in neurosciences and cancer research.

Demonstrations and practical sections in the laboratory.

Language of instruction

English

Mode of study

Not applicable.

Entry knowledge

Knowledge of geometric optics.

Rules for evaluation and completion of the course

Course finalizes by evaluation of the participation.

Aims

The objective of the course is the introduction of biophotonics, and light microscopy techniques for biology. The course also includes the basics of cell biology and the application of light microscopy in studies of live cells in vitro, and example applications in neurosciences and cancer research.
Acquiring of widened ability to interdisciplinary professional activity.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

A. R. Hibbs: Confocal Microscopy for Biologists. Springer, 2004
B. Alberts et al.: Molecular Biology of the Cell, 5th Ed. Garland Science, 2007
D. Bray: Cell Movements: From Molecules to Motility. Garland Science 2000
D. E. Chandler, R. W. Robertson: Bioimaging. Jones & Bartlett, Boston 2009

Recommended reading

Not applicable.