Introduction to light microscopy (historical overview completed by substantial pieces of knowledge of geometric and wave optics, light-microscope optical setup, techniques of light microscopy, and practical knowledge), theoretical description of image formation (wave theory of image formation based on the Abbe theory), fluorescence microscopy, confocal microscopy, interference and holographic microscopy (principle, setup of the device, imaging properties), X-ray computed tomography, spectroscopic methods (Laser-Induced Breakdown, UV, VIS, Raman), polarisation microscopy, computers and cameras in microscopy.

Demonstrations and practical exercises on light microscopy, and spectroscopy and particle spectroscopy are carried out in laboratories.