Course detail

Chromatography

FCH-MC_CHRAcad. year: 2025/2026

History of chromatography. Basic physical-chemical principles of chromatography. Liquid chromatography - individual techniques based on different separation mechanisms, instrumentation, applications, current trends. Gas chromatography - individual techniques based on different separation mechanisms, instrumentation, applications, current trends. Interpretation of chromatographic data, qualitative and quantitative analysis.

Language of instruction

Czech

Number of ECTS credits

4

Mode of study

Not applicable.

Guarantor

Ing. Ludmila Mravcová, Ph.D.

Department

Institute of Chemistry and Technology of Environmental Protection (ÚCHTOŽP)

Entry knowledge

Thorough knowledge of physical chemistry, good knowledge of common, inorganic, organic and analytical chemistry.

Rules for evaluation and completion of the course

Oral examination covering the range of discussed topics
Attendance at lectures is optional and is not controlled, but it is recommended.

Aims

The aim of this course is:
- Thorough familiarization of students with chromatography as an analytical separation technique
- detailed discussion of chromatographic separation mechanisms, including their capabilities and limitations
- introduction of students to the field of chromatography application in analytical practice
Successful completion of the course the student will gain the following knowledge and skills:
1. Students will be familiarized with the theory of chromatographic separation process and they will be able to apply theoretical knowledge in optimization of chromatographic separation
2. They will be provided with knowledge of instrumentation in liquid, gas and supercritical fluid chromatography
3. They will be able to assess the suitability of chromatographic techniques for solving specific analytical problems and critically evaluate the results.

Study aids

Not applicable.

Prerequisites and corequisites

Not applicable.

Basic literature

Churáček J. a kol.: Analytická separace látek. STNL Praha, 1990 (CS)
Churáček J. a kol.: Nové trendy v teorii a instrumentaci vybraných analytických metod. SNTL Praha, 1993. (CS)
L. Nováková, M. Douša: Moderní HPLC v teorii a praxi I, 2013 (CS)
L. Nováková, M. Douša: Moderní HPLC v teorii a praxi II, 2013 (CS)
V. R. Meyer: Practical High-Performance. Liquid Chromatography, 2004 (EN)

Recommended reading

Not applicable.

Classification of course in study plans

  • Programme NKCP_ECHT Master's 2 year of study, winter semester, compulsory-optional
  • Programme NPCP_ECHT Master's 2 year of study, winter semester, compulsory-optional

Type of course unit

 

Lecture

26 hod., optionally

Teacher / Lecturer

Ing. Ludmila Mravcová, Ph.D.

Syllabus

1. Introduction, history of chromatography, classification of chromatographic techniques
2. Basic theory of chromatography – thermodynamical and kinetic aspects, effectivity of chromatographic separation
3. Planar chromatography
4. Column chromatography – basic configuration and theory
5. High performance liquid chromatography – separation principles, instrumentation, columns and rules of their selection, normal and reversed-phase systems, isocratic and gradient elution, ion chromatography, ion-pair chromatography, hydrophobic interaction liquid chromatography, size exclusion chromatography, bioaffinity chromatography, current trends – UPLC, UHPLC
6. Gas chromatography – separation principles, instrumentation, capillary and packed columns, rules for their selection, classification of stationary phases in gas chromatography, derivatization in gas chromatography
7. Tandem techniques in chromatography – systems LC-LC, LC-GC, LC-ICP
8. Tandem techniques in chromatography – systems GC-GC, GCxGC
9. Fast chromatography
10. Qualitative aspects of chromatographic data – correlation structure-retention, retention indices in gas chromatography
11. Quantitation in chromatography
12. Troubleshooting in LC, practical examples
13. Troubleshooting in GC, practical examples

Guided consultation in combined form of studies

26 hod., optionally

Teacher / Lecturer

Ing. Ludmila Mravcová, Ph.D.