Detail předmětu

Biochemistry II

FCH-MA_BCH2Ak. rok: 2021/2022

Metabolic pathways. Bioenergetics and oxidative metabolism. Short-term and long-term control mechanisms - regulation of enzyme activity, gene expression regulation; cell signaling. Carbohydrate metabolism - major metabolic pathways and their control, special pathways. Tricarboxylic acid cycle. Electron transport chain. Oxidative phosphorylation. Lipid metabolism - utilization and storage of energy. Metabolismus of nitrogen compounds I - Amino acid metabolism. Metabolsim of nitrogen compounds II - Porfyrin and heme metabolism, Nucelotide metabolism. Metabolic interrelationships and control. Compartmentation. Membrane transport. Biochemistry of hormones and the hormonal cascade system.

Jazyk výuky

angličtina

Počet kreditů

4

Nabízen zahraničním studentům

Všech fakult

Výsledky učení předmětu

The course Biochemistry II is focused on detailed study of metabolic pathways and their regulation on molecular as well as cellular level. The knowlege obtained after passing the course can serve as basis for further studies, especially for biotechnology, molecular biology and bioengineering.

Prerekvizity

Examination - Biochemistry I.

Plánované vzdělávací činnosti a výukové metody

The course uses teaching methods in form of Lecture - 2 teaching hours per week. The e-learning system (LMS Moodle) is available to teachers and students.

Způsob a kritéria hodnocení

Oral examination, combination of 3 questions, evaluation according to ECTS standards.

Exercises: active participation in round discussions, individual presentation, 4 tests /10 questions, minimum points - 70 %.

Osnovy výuky

The aim of the course is to acquaint students with the basics of cellular metabolism and its regulation. Based on the general principles of biochemical thermodynamics, the basic pathways of metabolism of carbohydrates, fatty acids and amino acids will be discussed. The course also includes biological oxidation, cellular energy production and photosynthesis. Within the metabolism of nucleic acids and proteins, the course of gene expression and its regulation will be explained. The course also includes mutual relations and regulation within the intermediate metabolism of nutrients.
During exercises will be discussed individual topics presented in lectures. Round discussions will be realized and completed by indiviual presentations of students.

Week:
1. Basic principles of living systems, termodynamics, energy metabolism.
2. Control of metabolism: mechanisms of enzyme activity control (allostery, covalent modification); cell signaling.
3. Metabolic control 2: control of gene expressione; turnover of protein molecules.
4. Metabolism of saccharides – glycolysis, types of fermentation and their industrial use.
5. The tricarboxylic acid cycle. Gluconeogenesis. The ketone bodies.
6. Biological oxidations. Electron transport and oxidative phosphorylation – redox complexes, respiratory chain control.
7. Photosynthesis – principles, control (oxygenic, anoxygenic).
8. Metabolism of lipids: The beta-oxidation of fatty acids, FA biosynthesis. Metabolism of triacylglycerols and phospholipids.
9. The isoprenoid pathway. Cholesterol metabolism.
10. Metabolism of nitrogen compounds 1: nitrogen fixation, amino acids degradation. The urea cycle. Degradation of carbon chain of AA.. Biosynthesis of amino acids.
11. Metabolism of nitrogen compounds 2: Amino acids as biosynthetic precursors: The porphyrine pathway. Nucleotide metabolism. Biosynthesis and degradation of nucleotides and nucleic acids.
12. Secondary metabolism – overview.
13. Metabolism of selected classes of secondary metabolites – vitamins, antibiotics, aromatic compounds, alkaloids, glycosides.

Učební cíle

The aim of the course Biochemistry II is to introduce basic principles of cell metabolism and its regulation. Based on general thermodynamic principles the course of central biochemical pathways including metabolism of saccharides, fatty acids and amino acids is elucidated. Further, basic principles of biological oxidations, cell energy production and photosynthesis are discussed. Next, metabolism of nucleic acids and proteins and processes involved in gene expression are introduced. As a part of course intermediate metabolism and complex regulation of metabolic pathways are presented.

Vymezení kontrolované výuky a způsob jejího provádění a formy nahrazování zameškané výuky

Recommended.

Základní literatura

Stryer L.: Biochemistry. 9th Edition, Palgrave, 2019. (EN)
Voet D. (2011): Biochemistry, John Wiley And Sons Ltd, ISBN-13: 978-0470570951 (EN)

Doporučená literatura

Salway J.G.,Medical Biochemistry at a Glance, John Wiley And Sons Ltd, ISBN: 9780470654514 (EN)

Elearning

Zařazení předmětu ve studijních plánech

  • Program NPCP_CHMA magisterský navazující

    specializace BF , 1 ročník, zimní semestr, povinný
    specializace CHBL , 1 ročník, zimní semestr, povinný

  • Program NPCP_CHTPO magisterský navazující

    specializace PCH , 1 ročník, zimní semestr, povinný
    specializace CHPL , 1 ročník, zimní semestr, povinný

  • Program NKCP_CHTPO magisterský navazující

    specializace PCH , 1 ročník, zimní semestr, povinný
    specializace CHPL , 1 ročník, zimní semestr, povinný
    specializace BCH , 1 ročník, zimní semestr, povinný

  • Program NPCP_CHPL magisterský navazující 1 ročník, zimní semestr, povinný
  • Program NPAP_ENVI magisterský navazující 1 ročník, zimní semestr, povinný
  • Program NKCP_CHPL magisterský navazující 1 ročník, zimní semestr, povinný

Typ (způsob) výuky

 

Přednáška

26 hod., nepovinná

Vyučující / Lektor

Cvičení

13 hod., povinná

Vyučující / Lektor

Konzultace v kombinovaném studiu

26 hod., nepovinná

Vyučující / Lektor

Elearning