Detail předmětu

Advanced Techniques of Thermal Analysis

FCH-DA_PTTAk. rok: 2022/2023

Fluorescence spectroscopy. Fluorescence microscopy. Flow cytometry. Fluorescence correlation spectroscopy (FLIM, FCS). Technical aspects and interpretation of particular advanced fluorescence techniques. Preparation of model system (associative colloid system), analysis and data interpretation. Techniques: time resolved emission spectrocsopy, anisotrophy, fluorescence correlation spectroscopy, fluorescence bleaching, corss-correlation spectroscopy

Jazyk výuky

angličtina

Garant předmětu

doc. Ing. Petr Sedláček, Ph.D.

Zajišťuje ústav

Ústav fyzikální a spotřební chemie (ÚFSCH)

Způsob a kritéria hodnocení

Study results are verified pursuant to Article 34, Paragraph 7 of Study and Examination Rules of BUT. Students are given a credit for submitting protocols for each technique they studied. Protocols are processed in the following format: principles of the technique, the aim and exact methods used for measurement, collected data, their evaluation and interpretation, assessment of the impact of the technique on biophysical practice.

Osnovy výuky

Fluorescence spectroscopy. Fluorescence microscopy. Flow cytometry. Fluorescence correlation spectroscopy (FLIM, FCS). Technical aspects and interpretation of particular advanced fluorescence techniques. Preparation of model system (associative colloid system), analysis and data interpretation. Techniques: time resolved emission spectrocsopy, anisotrophy, fluorescence correlation spectroscopy, fluorescence bleaching, corss-correlation spectroscopy

Učební cíle

Fluorescence spectroscopy. Fluorescence microscopy. Flow cytometry. Fluorescence correlation spectroscopy (FLIM, FCS). Technical aspects and interpretation of particular advanced fluorescence techniques. Preparation of model system (associative colloid system), analysis and data interpretation. Techniques: time resolved emission spectrocsopy, anisotrophy, fluorescence correlation spectroscopy, fluorescence bleaching, corss-correlation spectroscopy

Základní literatura

Denes, L. The Nature of Biological Systems as Revealed by Thermal Methods (Hot Topics in Thermal Analysis and Calorimetry). Netherlands: Springer, 2005 (EN)
Privalov, P.L. Microcalorimetry of macromolecules: The Physical Basis of Biological Structures. N. York: Wiley, 2012 (EN)

Zařazení předmětu ve studijních plánech

  • Program DPAP_BCH doktorský 1 ročník, zimní semestr, povinně volitelný
  • Program DKAP_BCH doktorský 1 ročník, zimní semestr, povinně volitelný