Detail předmětu

Special Molecular Biotechnology

FCH-DA_SMBAk. rok: 2023/2024

The course focuses on the use of molecular biotechnology in the preparation of transgenic organisms for the isolation of biotechnologically important proteins. Attention is paid to the use of modern molecular biology and biophysical methods for their use in the production of significant proteins and the study of their interactions with DNA.

Jazyk výuky

angličtina

Garant předmětu

prof. Mgr. Václav Brázda, Ph.D.

Zajišťuje ústav

Ústav chemie potravin a biotechnologií (ÚCHPBT)

Pravidla hodnocení a ukončení předmětu

The results of the study are verified by colloquium (S.E.R. Art. 34 of the BUT Brno), ie a discussion about the subject matter connected with the preparation of a written work dealing with the specified topic of the subject in the range of about 10 text pages; the debate takes about 30 minutes.

Učební cíle

The aim of the course is to introduce students how to use transgenic organisms in the preparation of useful products, especially proteins. Students will get ready a review on the topic and will proceed all steps necessary to isolate the transgenic protein using the GATEWAY cloning system.

Základní literatura

Glick B. R., Pasternak J. J.: Molecular Biotechnology. ASM Press, Washington 2003. (EN)
https://www.thermofisher.com/cz/en/home/life-science/cloning/gateway-cloning.html (EN)
J. Čechová, J. Coufal, E.B. Jagelská, M. Fojta, V. Brázda, P73, Like Its P53 Homolog, Shows Preference for Inverted Repeats Forming Cruciforms, PLoS One. 13 (2018). doi:10.1371/journal.pone.0195835. (EN)
Rossana M., Genetic Engineering and Biotechnology, Callisto Reference, 2018 (EN)

Zařazení předmětu ve studijních plánech

  • Program DPAP_BCH doktorský 1 ročník, zimní semestr, povinně volitelný
  • Program DKAP_BCH doktorský 1 ročník, zimní semestr, povinně volitelný