2 hod., nepovinná

1. Introduction. Science of Nano. What is nanobiotechnology. 2. Nanostructures. Carbon nanotubes, semiconductor nanoparticles - quantum dots. Metal-based nanostructures - nanowires and bioelectronics. Gold nanoparticles (nanorods, nanocages, nanoshells). Magnetic nanoparticles. Polymer nanostructures (dendrimers). Protein-based nanostructures - nanomotors from microbes and mammalian cells (myosin). Nanomachines based on nucleic acids. 3. Experimental technichues. Scanning probe microscopies (STM, AFM, SNOM, SECM, ...). Physical principles, basic and advanced measuring modes. Imaging of bioobjects - from atoms, molecules to cells and tissues. Combined techniques with inverted optical and fluorescence microscopes. Raman imaging. Biointeractions at the molecular level. 4. Self-assembling techniques. Separation, characterization and modification of nanoparticles. From natural to artificial structures. Nanolithography and nanomanipulations. Nanoparticles for biological labeling and cellular imaging. Nanobiosensors and nanobioanalytical systems. Microfluidics, cell sorting and lab-on-a-chip. Biochips and sensing arrays, nanodeposition of biomolecules. 5. Medical applications. Cytotoxicity of nanoparticles. Nanostructures in drug discovery, delivery and controlled release. Nanostructures in cancer research. Nanotechnology for tissue engineering and regenerative therapy. 6. Nanobiotechnology in commercial examples. Perspectives and conclusions.