Detail publikace

Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours

VYSLOUŽILOVÁ, L. KŘÍŽKOVÁ, S. ANÝZ, J. HYNEK, D. HRABĚTA, J. KRUSEOVÁ, J. ECKSCHLAGER, T. ADAM, V. ŠTĚPÁNKOVÁ, O. KIZEK, R.

Originální název

Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

In this study, we determined serum levels of metallothioneins (MTs) and zinc in children with solid tumours (neuroblastoma, Hodgkin lymphoma, medulloblastoma, osteosarcoma, Ewing sarcoma and nephroblastoma) by differential pulse voltammetry Brdicka reaction and ELISA. Zn(II) level in patients sera was 40% compared to controls, contrariwise, MT level was 4.2 higher in patients. No significant differences among single diagnoses were found both for Zn(II) and MT. When determined Zn(II)/MT ratio, in controls its value was 24.6, but it was 2.6 in patients. After Western-blotting with anti-MT and anti-Zn chicken antibodies, variable intensities of the bands within the samples were observed. The brightness curve obtained for each sample both for MT- and Zn blots was further analysed to produce a list of band positions together with some complementary information related to the intensity of the observed bands by the optimised algorithm.

Klíčová slova

wavelet transformation, metallothionein, Western blot

Autoři

VYSLOUŽILOVÁ, L.; KŘÍŽKOVÁ, S.; ANÝZ, J.; HYNEK, D.; HRABĚTA, J.; KRUSEOVÁ, J.; ECKSCHLAGER, T.; ADAM, V.; ŠTĚPÁNKOVÁ, O.; KIZEK, R.

Rok RIV

2013

Vydáno

1. 6. 2013

ISSN

0173-0835

Periodikum

Electrophoresis

Ročník

34

Číslo

11

Stát

Spolková republika Německo

Strany od

1637

Strany do

1648

Strany počet

12

BibTex

@article{BUT101161,
  author="Lenka {Vysloužilová} and Soňa {Křížková} and Jiří {Anýz} and David {Hynek} and Jan {Hraběta} and Jarmila {Kruseová} and Tomáš {Eckschlager} and Vojtěch {Adam} and Olga {Štěpánková} and René {Kizek}",
  title="Use of brightness wavelet transformation for automated analysis of serum metallothioneins- and zinc-containing proteins by Western blots to subclassify childhood solid tumours",
  journal="Electrophoresis",
  year="2013",
  volume="34",
  number="11",
  pages="1637--1648",
  doi="10.1002/elps.201200561",
  issn="0173-0835"
}