Detail publikace

The importance of experimental milieu in regulation of macrophage activation

PEKAROVÁ, M. a kol.

Originální název

The importance of experimental milieu in regulation of macrophage activation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as simulated in vivo condition is of particular interest. Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-y, TNF-a and interleukins or their combination) under different experimental conditions (in vitro and simulated in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods. Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and simulated in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways. Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the classically used in vitro systems seems to be not suitable for correct analysis of their functions.

Klíčová slova

Macrophages, experimental milieu, activation, lipopolysacchride, Interferon-gamma

Autoři

PEKAROVÁ, M. a kol.

Rok RIV

2013

Vydáno

1. 5. 2013

Nakladatel

WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA

Místo

USA

ISSN

0014-2972

Periodikum

EUROPEAN JOURNAL OF CLINICAL INVESTIGATION

Ročník

43

Číslo

10

Stát

Spolková republika Německo

Strany do

10

Strany počet

10

BibTex

@article{BUT103124,
  author="Michaela {Pekarová}",
  title="The importance of experimental milieu in regulation of macrophage activation",
  journal="EUROPEAN JOURNAL OF CLINICAL INVESTIGATION",
  year="2013",
  volume="43",
  number="10",
  pages="10",
  issn="0014-2972"
}