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PEKAROVÁ, M. a kol.
Originální název
The importance of experimental milieu in regulation of macrophage activation
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Background: Macrophages are important source of cytokines and other compounds, which recruit additional cells to sites of infection or tissue injury. Importantly, their functions might be directly controlled by the composition of milieu in their immediate vicinity. Therefore, the analysis of their function under different in vitro as well as simulated in vivo condition is of particular interest. Materials and methods: Mice peritoneal macrophages were stimulated with different activators (e.g. LPS, IFN-y, TNF-a and interleukins or their combination) under different experimental conditions (in vitro and simulated in vivo conditions). Consequently, the physiological functions of macrophages were assessed using different luminometric, spectrophotometric, molecular, and immunohistochemical methods. Results: Our data demonstrate that there exists crucial difference in activation of macrophages under classical in vitro and simulated in vivo conditions. These abnormalities are accompanied by significant changes in inflammatory response of macrophages as well as in activation of intracellular signaling pathways. Conclusions: The activation of mice peritoneal macrophages is dependent on the composition of milieu in their immediate vicinity and the classically used in vitro systems seems to be not suitable for correct analysis of their functions.
Klíčová slova
Macrophages, experimental milieu, activation, lipopolysacchride, Interferon-gamma
Autoři
Rok RIV
2013
Vydáno
1. 5. 2013
Nakladatel
WILEY-BLACKWELL, 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
Místo
USA
ISSN
0014-2972
Periodikum
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION
Ročník
43
Číslo
10
Stát
Spolková republika Německo
Strany do
Strany počet
BibTex
@article{BUT103124, author="Michaela {Pekarová}", title="The importance of experimental milieu in regulation of macrophage activation", journal="EUROPEAN JOURNAL OF CLINICAL INVESTIGATION", year="2013", volume="43", number="10", pages="10", issn="0014-2972" }