Detail publikace

DNA interaction with zinc(II) ions

NEJDL, L. RUTTKAY-NEDECKÝ, B. KUDR, J. KŘÍŽKOVÁ, S. ŠMERKOVÁ, K. DOSTÁLOVÁ, S. VACULOVIČOVÁ, M. KOPEL, P. ZEHNÁLEK, J. TRNKOVÁ, L. BABULA, P. ADAM, V. KIZEK, R.

Originální název

DNA interaction with zinc(II) ions

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

We focused on interactions of Zn(II) with DNA in this study. These interactions were monitored usingUV/vis spectrophotometry and gel electrophoresis. Firstly, we isolated and amplified 498 bp fragmentof DNA. Samples were obtained by incubation of DNA fragment with Zn(II) for 60 min at 25 C. After incubation, the samples were dialyzed and analyzed immediately. In this way, DNA was converted intoa metal bound DNA (Zn–DNA). Interaction of Zn(II) with DNA caused change in the absorption spectrum(190–350 nm) and decrease in the melting temperature (Tm) of Zn–DNA. Spectrophotometric (UV/vis)analysis showed that increasing concentrations of zinc(II) ions led to the increase in the absorbance at200 nm and decrease in absorbance at 251 nm.

Klíčová slova

UV/vis spectrophotometry, Gel electrophoresis, Melting temperature

Autoři

NEJDL, L.; RUTTKAY-NEDECKÝ, B.; KUDR, J.; KŘÍŽKOVÁ, S.; ŠMERKOVÁ, K.; DOSTÁLOVÁ, S.; VACULOVIČOVÁ, M.; KOPEL, P.; ZEHNÁLEK, J.; TRNKOVÁ, L.; BABULA, P.; ADAM, V.; KIZEK, R.

Rok RIV

2014

Vydáno

1. 3. 2014

ISSN

0141-8130

Periodikum

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Ročník

64

Číslo

3

Stát

Nizozemsko

Strany od

281

Strany do

287

Strany počet

7

BibTex

@article{BUT106908,
  author="Lukáš {Nejdl} and Branislav {Ruttkay-Nedecký} and Jiří {Kudr} and Soňa {Křížková} and Kristýna {Šmerková} and Simona {Dostálová} and Markéta {Vaculovičová} and Pavel {Kopel} and Josef {Zehnálek} and Libuše {Trnková} and Petr {Babula} and Vojtěch {Adam} and René {Kizek}",
  title="DNA interaction with zinc(II) ions",
  journal="INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES",
  year="2014",
  volume="64",
  number="3",
  pages="281--287",
  doi="10.1016/j.ijbiomac.2013.12.013",
  issn="0141-8130"
}