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AUTRATA, R. NEDĚLA, V. HORKÝ, D.
Originální název
Influence of water environment on biological specimens imaging in ESEM
Typ
článek ve sborníku ve WoS nebo Scopus
Jazyk
angličtina
Originální abstrakt
Environmental scanning electron microscope (ESEM) allows the visualisation of specimens that are difficult or impossible to image in a conventional high vacuum SEM. Pressure in the specimen chamber ranges from 1 Pa over 2000 Pa, which permits to observe specimens of beeing free from charging artefacts and containing different volume of water. But there are some difficulties with the observation of highly wet biological specimens in their natural state because during a pre-pumping process of the specimen chamber an escape of water from the specimen occurs and thus its surface is damaged. The aim of this contribution is to find a method making possible to decrease water escape from the specimen during the initiation pumping process of the ESEM, immediately after the inserting of the specimen into the specimen chamber and during an image recording.
Klíčová slova
biological specimens, imaging, EREM
Autoři
AUTRATA, R.; NEDĚLA, V.; HORKÝ, D.
Vydáno
1. 1. 2003
Místo
Pula
Strany od
497
Strany do
498
Strany počet
2
URL
ÚPT AV ČR Brno
BibTex
@inproceedings{BUT10735, author="Rudolf {Autrata} and Vilém {Neděla} and Drahomír {Horký}", title="Influence of water environment on biological specimens imaging in ESEM", booktitle="Proceedings 6th Multinational Congress on Microscopy", year="2003", number="1", pages="2", address="Pula", url="ÚPT AV ČR Brno" }