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BEDNAŘÍK, A. KUBA, P. MOSKOVETS, E. TOMALOVÁ, I. KRÁSENSKÝ, P. HOUŠKA, P. PREISLER, J.
Originální název
Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) imaging of surfaces and tissues is a rapidly evolving technique having great potential in the field of biosciences. In earlier times, acquisition of a single high-resolution MS image could take days. Despite the recent introduction of high-repetition rate lasers to increase sample throughput of axial TOF MS instruments, obtaining a high-resolution image still requires a few hours. This paper shows that a substantial increase in the throughput of the TOF MS-based tissue imaging can be achieved by incorporating a mirror providing high-speed precision scanning of the laser beam along the sample surface. Equipped with the scanning mirror, a laboratory-built axial MALDI TOF MS instrument utilizing a 4-kHz UV laser recorded a 100 x 100 pixel MS image in -11 min using 100 laser shots per pixel. This is almost an order of magnitude faster when compared to a modern commercial instrument equipped with 1-kHz laser.
Klíčová slova
mass spectrometry imaging; laser beam scanning; MALDI; TOF; high-throughput
Autoři
BEDNAŘÍK, A.; KUBA, P.; MOSKOVETS, E.; TOMALOVÁ, I.; KRÁSENSKÝ, P.; HOUŠKA, P.; PREISLER, J.
Rok RIV
2014
Vydáno
21. 1. 2014
Nakladatel
American Chemical Society
Místo
Washington
ISSN
0003-2700
Periodikum
ANALYTICAL CHEMISTRY
Ročník
86
Číslo
2
Stát
Spojené státy americké
Strany od
982
Strany do
986
Strany počet
5
BibTex
@article{BUT109667, author="Antonín {Bednařík} and Jan {Preisler} and Iva {Tomalová} and Eugene {MOSKOVETS} and Pavel {Krásenský} and Pavel {Houška} and Pavel {Kuba}", title="Rapid Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging with Scanning Desorption Laser Beam", journal="ANALYTICAL CHEMISTRY", year="2014", volume="86", number="2", pages="982--986", doi="10.1021/ac402823n", issn="0003-2700" }