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ZIKMUND, T. KVASNICA, L. TÝČ, M. KŘÍŽOVÁ, A. ČOLLÁKOVÁ, J. CHMELÍK, R.
Originální název
Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
Transmitted light holographic microscopy is particularly used for quantitative phase imaging of transparent microscopic objects such as living cells. The study of the cell is based on extraction of the dynamic data on cell behaviour from the time-lapse sequence of the phase images. However, the phase images are affected by the phase aberrations that make the analysis particularly difficult. This is because the phase deformation is prone to change during long-term experiments. Here, we present a novel algorithm for sequential processing of living cells phase images in a time-lapse sequence. The algorithm compensates for the deformation of a phase image using weighted least-squares surface fitting. Moreover, it identifies and segments the individual cells in the phase image.All these procedures are performed automatically and applied immediately after obtaining every single phase image. This property of the algorithm is important for real-time cell quantitative phase imaging and instantaneous control of the course of the experiment by playback of the recorded sequence up to actual time. Such operators intervention is a forerunner of process automation derived from image analysis. The efficiency of the propounded algorithm is demonstrated on images of rat fibrosarcoma cells using an off-axis holographic microscope.
Klíčová slova
Aberration compensation, cell tracking, digital image processing, quantitative phase imaging, real-time holography.
Autoři
ZIKMUND, T.; KVASNICA, L.; TÝČ, M.; KŘÍŽOVÁ, A.; ČOLLÁKOVÁ, J.; CHMELÍK, R.
Rok RIV
2014
Vydáno
1. 11. 2014
ISSN
0022-2720
Periodikum
Journal of Microscopy
Ročník
256
Číslo
2
Stát
Spojené království Velké Británie a Severního Irska
Strany od
117
Strany do
125
Strany počet
9
BibTex
@article{BUT109829, author="Tomáš {Zikmund} and Lukáš {Kvasnica} and Matěj {Týč} and Aneta {Křížová} and Jana {Čolláková} and Radim {Chmelík}", title="Sequential processing of quantitative phase images for the study of cell behaviour in real-time digital holographic microscopy", journal="Journal of Microscopy", year="2014", volume="256", number="2", pages="117--125", doi="10.1111/jmi.12165", issn="0022-2720" }