Characterization of Egg Laying Hen and Broiler Fecal Microbiota in Poultry Farms in Croatia, Czech Republic, Hungary and Slovenia

článek v časopise ve Web of Science, Jimp

angličtina

Poultry meat is the most common protein source of animal origin for humans. However, intensive breeding of animals in confined spaces has led to poultry colonisation by microbiota with a zoonotic potential or encoding antibiotic resistances. In this study we were therefore interested in the prevalence of selected antibiotic resistance genes and microbiota composition in feces of egg laying hens and broilers originating from 4 different Central European countries determined by real-time PCR and 16S rRNA gene pyrosequencing, respectively. strA gene was present in 1 out of 10,000 bacteria. The prevalence of sul1, sul2 and tet(B) in poultry microbiota was approx. 6 times lower than that of the strA gene. tet(A) and cat were the least prevalent being present in around 3 out of 10,000,000 bacteria forming fecal microbiome. The core chicken fecal microbiota was formed by 26 different families. Rather unexpectedly, representatives of Desulfovibrionaceae and Campylobacteraceae, both capable of hydrogen utilisation in complex microbial communities, belonged among core microbiota families. Understanding the roles of individual population members in the total metabolism of the complex community may allow for interventions which might result in the replacement of Campylobacteraceae with Desulfovibrionaceae and a reduction of Campylobacter colonisation in broilers, carcasses, and consequently poultry meat products.

metagenomics, chicken microbiota, 16S rRNA

VÍDEŇSKÁ, P.; RAHMAN, M.; FALDYNOVÁ, M.; BABÁK, V.; MATULOVÁ, M.; PRUKNER-RADOVCIC, E.; KŘÍŽEK, I.; SMOLE-MOZINA, S.; KOVAC, J.; SZMOLKA, A.; NAGY, B.; SEDLÁŘ, K.; ČEJKOVÁ, D.; RYCHLÍK, I.

2014

16. 10. 2014

Public Library of Science

California (US)

1932-6203

PLOS ONE

9

10

Spojené státy americké

e110076

e110076

8

http://europepmc.org/articles/PMC4199679?pdf=render

http://hdl.handle.net/11012/69201