Detail publikace

Light Scattering Techniques Applied for the Study of Aging of Biopolymers and Biocolloids

KALINA, M. SMILEK, J. KLUČÁKOVÁ, M.

Originální název

Light Scattering Techniques Applied for the Study of Aging of Biopolymers and Biocolloids

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Biopolymers and biocolloids as natural product are often subjected to aggregation and degradation. Both these processes are extremely important and must be taken into account in the case of industrial application of these natural substances. Aggregated or degraded material used as a raw material for industrial production can significantly change the final yield and properties of product. This contribution is discussing the application of light scattering techniques for observation of stability of these compounds in time and for study of influence of different parameters (UV light, temperature, pH, ionic strength) on the stability. In experimental work proteins (bovine serum albumin, cystein) and polysaccharides (chitosan, glucose, sucrose, carboxymethylcellulose and sodium alginate) were used as model biopolymers. Biocolloids were represented by humic acids. Stability and degradation of proteins and polysaccharides was studied using zeta potential (method of electrophoretic light scattering) and molecular weight determination (combination of size exclusion chromatography with multiangle light scattering detection). Aging of humic acids was investigated by means of zeta potential differences in time and time development of particle size (method of dynamic light scattering). Results showed that light scattering techniques can be used as easy and quick methods for purposes of detection of the aggregation or degradation of biomaterials. These methods can be useful either in the case of checking of stored stock solutions, for continual controlling of raw materials for industrial production and also for detection of any deviations of final products.

Klíčová slova

light scattering, biopolymers, biocolloids, particle size, zeta potential, molecular weight, aggregation, degradation

Autoři

KALINA, M.; SMILEK, J.; KLUČÁKOVÁ, M.

Vydáno

28. 10. 2014

Nakladatel

Masaryk University

Místo

Brno

ISBN

978-80-210-7159-9

Kniha

CEITEC Annual Conference - "Frontiers in Materials and Life Sciences"

Strany od

143

Strany do

143

Strany počet

1

BibTex

@misc{BUT110153,
  author="Michal {Kalina} and Jiří {Smilek} and Martina {Klučáková}",
  title="Light Scattering Techniques Applied for the Study of Aging of Biopolymers and Biocolloids",
  booktitle="CEITEC Annual Conference - {"}Frontiers in Materials and Life Sciences{"}",
  year="2014",
  pages="143--143",
  publisher="Masaryk University",
  address="Brno",
  isbn="978-80-210-7159-9",
  note="abstract"
}