Detail publikace

Time-Resolved Fluorescence Spectra of Different Soil Humic Acids and Standard IHSS Elliott Soil

ENEV, V. KLUČÁKOVÁ, M. DOSKOČIL, L.

Originální název

Time-Resolved Fluorescence Spectra of Different Soil Humic Acids and Standard IHSS Elliott Soil

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

The aim of this work was study structure and dynamics of different soil humic acids (HAs). Object of our study were three samples HAs which were isolated from sandy soil – Arenosols (locality Ratíškovice, Czech Republic), Chernozem – Haplic Luvisol (locality Praha-Ruzyně, Crop Research Institute, Czech Republic) and standard HA Elliott Soil (1S102H). Isolation of soil HAs were performed according to the procedure recommended by the International Humic Substances Society (IHSS). All samples of soil HAs were characterized by Steady-State and Time-Resolved Fluorescence Spectroscopy. Emission wavelength dependent fluorescence decays are used to construct Time-Resolved Fluorescence Spectra (TRES) of soil humic acids, which are useful to obtained information on the excited state kinetics and heterogeneity of emissive species in a system. Time resolved fluorescence spectra (TRES), plotted as fluorescence intensity vs. wavelength, were constructed using alfai (wavelength) and taui (wavelength), and steady-state emission spectrum corrected for the quantum efficiency of the photomultiplier. Fluorescence spectra were recorded in aqueous solutions of 10 mg/L HAs after overnight equilibration at room temperature, using FluoroLog luminescence spectrophotometer. The pH-value of the samples was adjusted to seven using a standard phosphate buffer. Steady-State emission spectra were recorded over the range of 390–600 nm at a constant excitation wavelength of 329 nm. Time-resolved fluorescence measurements were carried out by time-correlated single-photon counting (TCSPC) method. Fluorescence decay at each wavelength was deconvoluted using the instrument response function and a multiexponential function (three exponential functions). The fluorescence intensity values (in counts per second – CPS) of samples were corrected using method of Lakowicz.

Klíčová slova

Soil Humic Acids, Time-Resolved Fluorescence Spectroscopy, Steady-State Fluorescence Spectroscopy, Time-Resolved Emission Spectra (TRES), Bathochromic Shift of Fluorescence Maximum

Autoři

ENEV, V.; KLUČÁKOVÁ, M.; DOSKOČIL, L.

Vydáno

5. 11. 2014

Nakladatel

TANGER Ltd., Keltičkova 62, 710 00, Ostrava, Česká Republika, EU

Místo

AMOS repro Ltd., Čs. Legií 8, 702 00 Ostrava, Česká republika, EU

ISBN

978-80-87294-55-0

Kniha

Conference Proceedings, 6th International Conference November 5th–7th 2014, Hotel Voronez 1, Brno, Czech Republic, EU

Edice

2014 TANGER Ltd., Ostrava

Číslo edice

1st Edition, 2014

Strany od

168

Strany do

169

Strany počet

2

BibTex

@misc{BUT110283,
  author="Vojtěch {Enev} and Martina {Klučáková} and Leoš {Doskočil}",
  title="Time-Resolved Fluorescence Spectra of Different Soil Humic Acids and Standard IHSS Elliott Soil",
  booktitle="Conference Proceedings, 6th International Conference November 5th–7th 2014, Hotel Voronez 1, Brno, Czech Republic, EU",
  year="2014",
  series="2014 TANGER Ltd., Ostrava",
  edition="1st Edition, 2014",
  pages="168--169",
  publisher="TANGER Ltd., Keltičkova 62, 710 00, Ostrava, Česká Republika, EU",
  address="AMOS repro Ltd., Čs. Legií 8, 702 00 Ostrava, Česká republika, EU",
  isbn="978-80-87294-55-0",
  note="abstract"
}