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Čuta R., Fričová M., Rittich B., Španová A.
Originální název
TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA
Typ
konferenční sborník (ne článek)
Jazyk
angličtina
Originální abstrakt
The aim of this work was the optimization of procedure the crude cell lysates preparation of Lactobacillus genus. In this experiment different lysis methods were tested: buffer B, 4% solution of washing powder Amway and using of immobilized lysozyme. At the same time a lysing reagent was tested. As a control sample was used a buffer A which contained no lysozyme. Crude lysates were used for a DNA isolation using magnetic microspheres. The DNA was examined by the PCR method.
Klíčová slova
DNA, lysis, PCR
Autoři
Vydáno
1. 10. 2014
ISBN
978-80-904959-2-0
Strany od
170
Strany do
171
Strany počet
2
BibTex
@proceedings{BUT111852, editor="Robert {Čuta} and Michaela {Čutová} and Bohuslav {Rittich} and Alena {Španová}", title="TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA", year="2014", pages="170--171", isbn="978-80-904959-2-0" }