Detail publikace

UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed

ČUMOVÁ, M. ČÁSLAVSKÝ, J. KOSUBOVÁ, P., POSPÍCHALOVÁ, M.

Originální název

UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

Ergot alkaloids are mycotoxins produced predominantly by fungi of the Claviceps genus, which mainly affect cereals and wild grasses. Ingestion of ergot alkaloids can cause severe health problems in humans and animals. Thus, there is a need for methods that allow determination of these dangerous toxins. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of eight ergot alkaloids: ergosine, ergocornine, ergocryptine and ergocristine and their corresponding epimers as well. Mean recovery, precision, matrix effects and limits of quantification (LOQ) have been assessed for cereal and grass matrices within the method validation. Sample preparation has been based on modified QuEChERS approach. The presented method was used to inspect various feed samples. Ergot alkaloids were found in 31 out of 113 samples investigated. Ergocornine was the most frequently occuring alkaloid, while the highest levels were observed for ergocryptine, ergocristinine and ergosinine, depending on the product type. The total alkaloid content in positive samples varied from 10.4 µg/kg to 1855 µg/kg.

Klíčová slova

alkaloids, ergot alkaloids, feed

Autoři

ČUMOVÁ, M.; ČÁSLAVSKÝ, J.; KOSUBOVÁ, P., POSPÍCHALOVÁ, M.

Rok RIV

2014

Vydáno

6. 12. 2014

Nakladatel

VUT Brno

Místo

Brno

ISBN

978-80-214-5073-8

Kniha

EMEC15 Book of Abstracts

Strany od

113

Strany do

113

Strany počet

523

BibTex

@inproceedings{BUT111924,
  author="Martina {Čumová} and Josef {Čáslavský} and Petra {Kosubová}",
  title="UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed",
  booktitle="EMEC15 Book of Abstracts",
  year="2014",
  pages="113--113",
  publisher="VUT Brno",
  address="Brno",
  isbn="978-80-214-5073-8"
}