Detail publikace

Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection

KONEČNÁ, R. NGUYEN, H. STANISAVLJEVIC, M. BLAŽKOVÁ, I. KŘÍŽKOVÁ, S. VACULOVIČOVÁ, M. STIBOROVÁ, M.

Originální název

Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Doxorubicin (DOX) belongs to the group of anthracycline antibiotics with very effective anticancer properties. On the other hand, the cardiotoxic effects limit its application over the maximum cumulative dose. To overcome this obstacle, encapsulation of this drug into the protective nanotransporter such as apoferritin is beneficial. In this study, fluorescent behavior of DOX in various solvents was determined by fluorescence spectrometry, demonstrating the fluorescence quenching effect of water, which is often used as a solvent. It was found that by increasing the amount of the organic phase in the DOX solvent the dynamic quenching is significantly suppressed. Ethanol, acetonitrile and dimethyl sulfoxide were tested and the best linearity of the calibration curve was obtained when above 50 % of the solvent was present in the binary mixture with water. Moreover, pH influence on the DOX fluorescence was also observed within the range of 4–10. Two times higher fluorescence intensity was observed at pH 4 compared to pH 10.

Klíčová slova

Capillary electrophoresis, Fluorescence, Drug delivery, Doxorubicin, Apoferritin

Autoři

KONEČNÁ, R.; NGUYEN, H.; STANISAVLJEVIC, M.; BLAŽKOVÁ, I.; KŘÍŽKOVÁ, S.; VACULOVIČOVÁ, M.; STIBOROVÁ, M.

Rok RIV

2014

Vydáno

1. 11. 2014

ISSN

0009-5893

Periodikum

CHROMATOGRAPHIA

Ročník

77

Číslo

21-22

Stát

Spolková republika Německo

Strany od

1469

Strany do

1476

Strany počet

8

BibTex

@article{BUT112437,
  author="Romana {Konečná} and Hoai Viet {Nguyen} and Maja {Stanisavljevic} and Iva {Blažková} and Soňa {Křížková} and Markéta {Vaculovičová} and Marie {Stiborová}",
  title="Doxorubicin Encapsulation Investigated by Capillary Electrophoresis with Laser- Induced Fluorescence Detection",
  journal="CHROMATOGRAPHIA",
  year="2014",
  volume="77",
  number="21-22",
  pages="1469--1476",
  doi="10.1007/s10337-014-2733-6",
  issn="0009-5893"
}