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KUDR, J. NEJDL, L. SKALIČKOVÁ, S. ŽŮREK, M. MILOSAVLJEVIĆ, V. KENŠOVÁ, R. RUTTKAY-NEDECKÝ, B. KOPEL, P. HYNEK, D. NOVOTNÁ, M. ADAM, V. KIZEK, R.
Originální název
Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The aim of this study was to synthesize cadmium telluride nanoparticles (CdTe NPs) modified apoferritin, and examine if apoferritin is able to accommodate CdTe NPs. Primarily, the thermostability of horse spleen apoferritin was tested and it's unfolding at 70 C was observed. Cadmium telluride nanoparticles (CdTe NPs) were synthesized both within apoferritin protein cage and on its surface. The thermal treatment of apoferritin with CdTe NPs resulted in the aggregation of cores, which was indicated by changes in the absorption spectra and the shape of apoferritin tryptophan fluorescence. The apoferritin modified with CdTe NPs was additionally modified with gold nanoparticles and attached to magnetic particles via oligonucleotide using gold affinity to thiol group. This anchor system was used to separate the construct using external magnetic field and to analyse the molecules attached to apoferritin.
Klíčová slova
MODIFIED MAGNETIC PARTICLES; HORSE SPLEEN APOFERRITIN; BOVINE SERUM-ALBUMIN; CAGE-SHAPED PROTEIN; CDTE QUANTUM DOTS; GOLD NANOPARTICLES; BIOMIMETIC SYNTHESIS; THERMAL-STABILITY; CDS NANOPARTICLES; AQUEOUS-SOLUTIONS
Autoři
KUDR, J.; NEJDL, L.; SKALIČKOVÁ, S.; ŽŮREK, M.; MILOSAVLJEVIĆ, V.; KENŠOVÁ, R.; RUTTKAY-NEDECKÝ, B.; KOPEL, P.; HYNEK, D.; NOVOTNÁ, M.; ADAM, V.; KIZEK, R.
Rok RIV
2015
Vydáno
14. 3. 2015
ISSN
2050-750X
Periodikum
Journal of Materials Chemistry B
Ročník
3
Číslo
10
Stát
Spojené království Velké Británie a Severního Irska
Strany od
2109
Strany do
2118
Strany počet
BibTex
@article{BUT113985, author="Jiří {Kudr} and Lukáš {Nejdl} and Sylvie {Skaličková} and Michal {Žůrek} and Vedran {Milosavljević} and Renáta {Kenšová} and Branislav {Ruttkay-Nedecký} and Pavel {Kopel} and David {Hynek} and Marie {Novotná} and Vojtěch {Adam} and René {Kizek}", title="Use of nucleic acids anchor system to reveal apoferritin modification by cadmium telluride nanoparticles", journal="Journal of Materials Chemistry B", year="2015", volume="3", number="10", pages="2109--2118", doi="10.1039/c4tb01336k", issn="2050-750X" }