Detail publikace

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging

SLABÝ, T. KŘÍŽOVÁ, A. LOŠŤÁK, M. ČOLLÁKOVÁ, J. VESELÝ, P. CHMELÍK, R.

Originální název

Coherence-controlled holographic microscopy for live-cell quantitative phase imaging

Typ

článek ve sborníku ve WoS nebo Scopus

Jazyk

angličtina

Originální abstrakt

In this paper we present coherence-controlled holographic microscopy (CCHM) and various examples of observations of living cells including combination of CCHM with fluorescence microscopy. CCHM is a novel technique of quantitative phase imaging (QPI). It is based on grating off-axis interferometer, which is fully adapted for the use of incoherent illumination. This enables high-quality QPI free from speckles and parasitic interferences and lateral resolution of classical widefield microscopes. Label-free nature of QPI makes CCHM a useful tool for long-term observations of living cells. Moreover, coherence-gating effect induced by the use of incoherent illumination enables QPI of cells even in scattering media. Combination of CCHM with common imaging techniques brings the possibility to exploit advantages of QPI while simultaneously identifying the observed structures or processes by well-established imaging methods. We used CCHM for investigation of general parameters of cell life cycles and for research of cells reactions to different treatment. Cells were also visualized in 3D collagen gel with the use of CCHM. It was found that both the cell activity and movement of the collagen fibers can be registered. The method of CCHM in combination with fluorescence microscopy was used in order to obtain complementary information about cell morphology and identify typical morphological changes associated with different types of cell death. This combination of CCHM with common imaging technique has a potential to provide new knowledge about various processes and simultaneously their confirmation by comparison with known imaging method.

Klíčová slova

holographic microscope, quantitative phase imaging, interference microscope, live cell, fluorescence microscopy, digital holography, digital holographic microscopy, incoherent holography

Autoři

SLABÝ, T.; KŘÍŽOVÁ, A.; LOŠŤÁK, M.; ČOLLÁKOVÁ, J.; VESELÝ, P.; CHMELÍK, R.

Rok RIV

2015

Vydáno

11. 3. 2015

Nakladatel

SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

Místo

San Francisco, CA

ISBN

978-1-62841-426-4

Kniha

Quantitative Phase Imaging

Strany od

93360K-1

Strany do

93360K-4

Strany počet

4

URL