Detail publikace

SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA

FRIČOVÁ, M. ČUTA, R. KŘÍŽOVÁ, J. ŠPANOVÁ, A. RITTICH, B.

Originální název

SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA

Typ

abstrakt

Jazyk

angličtina

Originální abstrakt

Identification of bacterial DNA consists of several steps: cell lysis, extraction and purification of DNA followed by the identification of the bacterial species using polymerase chain reaction (PCR). Genomic DNA was isolated from Lactobacillus plantarum CCM 7039, Lactobacillus fermentum CCM 7192, Lactobacillus rhamnosus CCM 1825, Lactobacillus casei subsp. casei CCM 7088, and Bifidobacterium bifidum CCM 3762 by fenol extraction procedure and by the salting out with NaCl solution. Crude cell lysates were prepared using standard procedure and using of laundry powder. The quality of isolated DNA was verified by UV spectrophotometry and by agarose gel electrophoresis. Isolated DNA was used as a template for PCR

Klíčová slova

lacitc acid bacteria, DNA isolation, salting out procedure, PCR

Autoři

FRIČOVÁ, M.; ČUTA, R.; KŘÍŽOVÁ, J.; ŠPANOVÁ, A.; RITTICH, B.

Vydáno

26. 8. 2015

Nakladatel

Brno University of Technology, Faculty of Chemistry

Místo

Brno

ISBN

978-80-214-5228-2

Kniha

Book of abstracts

Edice

first

Strany od

98

Strany do

99

Strany počet

200

BibTex

@misc{BUT116125,
  author="FRIČOVÁ, M. and ČUTA, R. and KŘÍŽOVÁ, J. and ŠPANOVÁ, A. and RITTICH, B.",
  title="SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA",
  booktitle="Book of abstracts",
  year="2015",
  series="first",
  volume="2015",
  pages="98--99",
  publisher="Brno University of Technology, Faculty of Chemistry",
  address="Brno",
  isbn="978-80-214-5228-2",
  note="abstract"
}