Detail publikace

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

KOMÍNKOVÁ, M. HORKÝ, P. KIZEK, R. CERNEI, N. TMEJOVÁ, K. RUTTKAY-NEDECKÝ, B. GURÁŇ, R. POHANKA, M. ZÍTKA, O. ADAM, V.

Originální název

Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Taurine exhibits many positive effects on organism, such as induction of important protective biochemical mechanisms and antioxidant effects. In this work, we studied the effect of taurine consumption on levels of taurine, reduced glutathione (GSH) and metallothionein in liver and brain tissues of Wistar rats. The aim of the experiment was the monitoring of changes in concentration of taurine in brain and liver tissues and also changes in concentration of GSH and metallothionein. After optimization, the limit of detection of GSH in brain and liver tissue was established as 40 nmol.g-1 of protein and 90 nmol.g-1 of protein, respectively. That is sufficient for determination of the concentration alterations of GSH in studied tissues. The consumption of taurine increased content of GSH determined in the brain and liver (increased by approximately 0.8 mmol.g-1 of protein in comparison with control). The increase of concentration of taurine occurred only in the brain, by cca 50 μg.g-1 of tissue, regardless the applied concentration of taurine. The applied concentration of taurine had no effect on concentration of metallothionein in any of the examined tissue.

Klíčová slova

glutathione; taurine; metallothionein; antioxidant; amino acids; rats; HPLC

Autoři

KOMÍNKOVÁ, M.; HORKÝ, P.; KIZEK, R.; CERNEI, N.; TMEJOVÁ, K.; RUTTKAY-NEDECKÝ, B.; GURÁŇ, R.; POHANKA, M.; ZÍTKA, O.; ADAM, V.

Rok RIV

2014

Vydáno

30. 12. 2014

ISSN

1452-3981

Periodikum

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Ročník

10

Číslo

2

Stát

Srbská republika

Strany od

1716

Strany do

1727

Strany počet

12

BibTex

@article{BUT118726,
  author="Markéta {Komínková} and Pavel {Horký} and René {Kizek} and Natalia Vladimirovna {Cernei} and Kateřina {Tmejová} and Branislav {Ruttkay-Nedecký} and Roman {Guráň} and Miroslav {Pohanka} and Ondřej {Zítka} and Vojtěch {Adam}",
  title="Optimization of the Glutathione Detection by High Performance Liquid Chromatography with Electrochemical Detection in the Brain and Liver of Rats Fed with Taurine",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2014",
  volume="10",
  number="2",
  pages="1716--1727",
  issn="1452-3981"
}