Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
MANAKHOV, A. MAKHNEVA, E. SKLÁDAL, P. NEČAS, D. ČECHAL, J. KALINA, L. ELIÁŠ, M. ZAJÍČKOVÁ, L.
Originální název
The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiol-based self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications.
Klíčová slova
Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM
Autoři
MANAKHOV, A.; MAKHNEVA, E.; SKLÁDAL, P.; NEČAS, D.; ČECHAL, J.; KALINA, L.; ELIÁŠ, M.; ZAJÍČKOVÁ, L.
Vydáno
1. 1. 2016
ISSN
0169-4332
Periodikum
Applied Surface Science
Ročník
360
Číslo
Part A
Stát
Nizozemsko
Strany od
28
Strany do
36
Strany počet
9
BibTex
@article{BUT119199, author="Anton {Manakhov} and Ekaterina {Makhneva} and Petr {Skládal} and David {Nečas} and Jan {Čechal} and Lukáš {Kalina} and Marek {Eliáš} and Lenka {Zajíčková}", title="The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry", journal="Applied Surface Science", year="2016", volume="360", number="Part A", pages="28--36", doi="10.1016/j.apsusc.2015.10.178", issn="0169-4332" }