Přístupnostní navigace
E-přihláška
Vyhledávání Vyhledat Zavřít
Detail publikace
LEXA, M. ŠTEFLOVÁ, P. MARTÍNEK, T. VORLÍČKOVÁ, M. VYSKOT, B. KEJNOVSKÝ, E.
Originální název
Guanine quadruplexes are formed by specific regions of human transposable elements
Typ
článek v časopise ve Web of Science, Jimp
Jazyk
angličtina
Originální abstrakt
BACKGROUND:Transposable elements form a significant proportion of eukaryotic genomes. Recently, Lexa et al (Nucleic Acids Res 42:968-978, 2014) reported that plant long terminal repeat (LTR) retrotransposons often contain potential quadruplex sequences (PQSs) in their LTRs and experimentally confirmed their ability to adopt four-stranded DNA conformations.RESULTS:Here, we searched for PQSs in human retrotransposons and found that PQSs are specifically localized in the 3'-UTR of LINE-1 elements, in LTRs of HERV elements and are strongly accumulated in specific regions of SVA elements. Circular dichroism spectroscopy confirmed that most PQSs had adopted monomolecular or bimolecular guanine quadruplex structures. Evolutionarily young SVA elements contained more PQSs than older elements and their propensity to form quadruplex DNA was higher. Full-length L1 elements contained more PQSs than truncated elements; the highest proportion of PQSs was found inside transpositionally active L1 elements (PA2 and HS families).CONCLUSIONS:Conservation of quadruplexes at specific positions of transposable elements implies their importance in their life cycle. The increasing quadruplex presence in evolutionary young LINE-1 and SVA families makes these elements important contributors toward present genome-wide quadruplex distribution.
Klíčová slova
G4 quadruplex; human transposable elements; circular dichroism; Bioconductor code; bioinformatics
Autoři
LEXA, M.; ŠTEFLOVÁ, P.; MARTÍNEK, T.; VORLÍČKOVÁ, M.; VYSKOT, B.; KEJNOVSKÝ, E.
Rok RIV
2014
Vydáno
27. 11. 2014
ISSN
1471-2164
Periodikum
BMC GENOMICS
Ročník
15
Číslo
1032
Stát
Spojené království Velké Británie a Severního Irska
Strany od
1
Strany do
12
Strany počet
URL
http://www.biomedcentral.com/1471-2164/15/1032
BibTex
@article{BUT119812, author="Matej {Lexa} and Pavlína {Šteflová} and Tomáš {Martínek} and Michaela {Vorlíčková} and Boris {Vyskot} and Eduard {Kejnovský}", title="Guanine quadruplexes are formed by specific regions of human transposable elements", journal="BMC GENOMICS", year="2014", volume="15", number="1032", pages="1--12", doi="10.1186/1471-2164-15-1032", issn="1471-2164", url="http://www.biomedcentral.com/1471-2164/15/1032" }