Detail publikace

Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

KOLEK, J. SEDLÁŘ, K. PROVAZNÍK, I. PATÁKOVÁ, P.

Originální název

Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation

Typ

článek v časopise ve Web of Science, Jimp

Jazyk

angličtina

Originální abstrakt

Butanol is currently one of the most discussed biofuels. Its use provides many benefits in comparison to bio-ethanol, but the price of its fermentative production is still high. Genetic improvements could help solve many problems associated with butanol production during ABE fermentation, such as its toxicity, low concentration achievable in the cultivation medium, the need for a relatively expensive substrate, and many more. Clostridium pasteurianum NRRL B-598 is non-type strain producing butanol, acetone, and a negligible amount of ethanol. Its main benefits are high oxygen tolerance, utilization of a wide range of carbon and nitrogen sources, and the availability of its whole genome sequence. However, there is no established method for the transfer of foreign DNA into this strain; this is the next step necessary for progress in its use for butanol production.

Klíčová slova

Clostridium; Butanol; Transformation; Conjugation; Methylation; Dam; Dcm; Electroporation; Sonoporation

Autoři

KOLEK, J.; SEDLÁŘ, K.; PROVAZNÍK, I.; PATÁKOVÁ, P.

Vydáno

20. 1. 2016

Nakladatel

BioMed Central

ISSN

1754-6834

Periodikum

BIOTECHNOL BIOFUELS

Ročník

9

Číslo

1

Stát

Spojené království Velké Británie a Severního Irska

Strany od

1

Strany do

14

Strany počet

14

URL

http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0436-y

Plný text v Digitální knihovně

http://hdl.handle.net/11012/69198

BibTex

@article{BUT120980,
  author="Jan {Kolek} and Karel {Sedlář} and Valentine {Provazník} and Petra {Patáková}",
  title="Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation",
  journal="BIOTECHNOL BIOFUELS",
  year="2016",
  volume="9",
  number="1",
  pages="1--14",
  doi="10.1186/s13068-016-0436-y",
  issn="1754-6834",
  url="http://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-016-0436-y"
}